biostudies-literatureUnknown7(1)Wong CFDespite the highly oxidative environment of the phagosomal lumen, the need for maintaining redox homeostasis is a critical aspect of mycobacterial biology. The pathogens are equipped with the sophisticated thioredoxin- (Trx) and peroxiredoxin system, including TrxC and the alkyl hydroperoxide reductase subunit C (AhpC), whereby TrxC is one of the reducing partners of AhpC. Here we visualize the redox modulated dodecamer ring formation of AhpC from Mycobacterium bovis (BCG strain; MbAhpC) using electron microscopy and present novel insights into the unique N-terminal epitope (40 residues) of mycobacterial AhpC. Truncations and amino acid substitutions of residues in the unique N-terminus of MbAhpC provide insights into their structural and enzymatic roles, and into the evolutionary divergenScientific reports5159https://www.ebi.ac.uk/biostudies/studies/S-EPMC5505994biostudies-literatureAhpC of the mycobacterial antioxidant defense system and its interaction with its reducing partner Thioredoxin-C.PMC5505994Wong CFSaw WGBhushan SRagunathan PShin JGruber GSubramanian Manimekalai MSKumar AYin ZfalseAhpC of the mycobacterial antioxidant defense system and its interaction with its reducing partner Thioredoxin-C.Despite the highly oxidative environment of the phagosomal lumen, the need for maintaining redox homeostasis is a critical aspect of mycobacterial biology. The pathogens are equipped with the sophisticated thioredoxin- (Trx) and peroxiredoxin system, including TrxC and the alkyl hydroperoxide reductase subunit C (AhpC), whereby TrxC is one of the reducing partners of AhpC. Here we visualize the redox modulated dodecamer ring formation of AhpC from Mycobacterium bovis (BCG strain; MbAhpC) using electron microscopy and present novel insights into the unique N-terminal epitope (40 residues) of mycobacterial AhpC. Truncations and amino acid substitutions of residues in the unique N-terminus of MbAhpC provide insights into their structural and enzymatic roles, and into the evolutionary divergen2017-01-01T00:00:00Z2017 Jul2026-05-05T11:08:31.848Z2025-06-01T00:37:08.114ZS-EPMC55059942869856910.1038/s41598-017-05354-5