biostudies-literatureUnknown7(2)Grußer LDespite years of research, treatment of traumatic brain injury (TBI) remains challenging. Considerable data exists that some volatile anesthetics might be neuroprotective. However, several studies have also revealed a rather neurotoxic profile of anesthetics. In this study, we investigated the effects of argon 50%, desflurane 6% and their combination in an <i>in vitro</i> TBI model with incubation times similar to narcotic time slots in a daily clinical routine. Organotypic hippocampal brain slices of 5- to 7-day-old mice were cultivated for 14 days before TBI was performed. Slices were eventually incubated for 2 hours in an atmosphere containing no anesthetic gas, argon 50% or desflurane 6% or both. Trauma intensity was evaluated <i>via</i> fluorescent imagery. Our results show that neithMedical gas research93-100https://www.ebi.ac.uk/biostudies/studies/S-EPMC5510299biostudies-literatureArgon attenuates the emergence of secondary injury after traumatic brain injury within a 2-hour incubation period compared to desflurane: an <i>in vitro</i> study.PMC5510299Hollig AGrußer LBlaumeiser-Debarry RKremer BRossaint RCoburn MKrings MfalseArgon attenuates the emergence of secondary injury after traumatic brain injury within a 2-hour incubation period compared to desflurane: an <i>in vitro</i> study.Despite years of research, treatment of traumatic brain injury (TBI) remains challenging. Considerable data exists that some volatile anesthetics might be neuroprotective. However, several studies have also revealed a rather neurotoxic profile of anesthetics. In this study, we investigated the effects of argon 50%, desflurane 6% and their combination in an <i>in vitro</i> TBI model with incubation times similar to narcotic time slots in a daily clinical routine. Organotypic hippocampal brain slices of 5- to 7-day-old mice were cultivated for 14 days before TBI was performed. Slices were eventually incubated for 2 hours in an atmosphere containing no anesthetic gas, argon 50% or desflurane 6% or both. Trauma intensity was evaluated <i>via</i> fluorescent imagery. Our results show that neith2017-01-01T00:00:00Z2017 Apr-Jun2025-04-18T20:41:17.914Z2019-03-27T02:50:26ZS-EPMC55102992874436110.4103/2045-9912.208512