{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chitty JL"],"funding":["National Health and Medical Research Council"],"pagination":["11829-11839"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5512076"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["292(28)"],"pubmed_abstract":["There is significant clinical need for new antifungal agents to manage infections with pathogenic species such as <i>Cryptococcus neoformans</i> Because the purine biosynthesis pathway is essential for many metabolic processes, such as synthesis of DNA and RNA and energy generation, it may represent a potential target for developing new antifungals. Within this pathway, the bifunctional enzyme adenylosuccinate (ADS) lyase plays a role in the formation of the key intermediates inosine monophosphate and AMP involved in the synthesis of ATP and GTP, prompting us to investigate ADS lyase in <i>C. neoformans.</i> Here, we report that <i>ADE13</i> encodes ADS lyase in <i>C. neoformans.</i> We found that an <i>ade13</i>Δ mutant is an adenine auxotroph and is unable to successfully cause infection"],"journal":["The Journal of biological chemistry"],"pubmed_title":["<i>Cryptococcus neoformans</i> ADS lyase is an enzyme essential for virulence whose crystal structure reveals features exploitable in antifungal drug design."],"pmcid":["PMC5512076"],"funding_grant_id":["APP1049716","1003325, 1110971"],"pubmed_authors":["Chitty JL","Kappler U","Butler MS","Fraser JA","Blake KL","Thompson M","Koh YQAE","Robertson AAB","Williams SJ","Blundell RD","Cooper MA","Kobe B"],"additional_accession":[]},"is_claimable":false,"name":"<i>Cryptococcus neoformans</i> ADS lyase is an enzyme essential for virulence whose crystal structure reveals features exploitable in antifungal drug design.","description":"There is significant clinical need for new antifungal agents to manage infections with pathogenic species such as <i>Cryptococcus neoformans</i> Because the purine biosynthesis pathway is essential for many metabolic processes, such as synthesis of DNA and RNA and energy generation, it may represent a potential target for developing new antifungals. Within this pathway, the bifunctional enzyme adenylosuccinate (ADS) lyase plays a role in the formation of the key intermediates inosine monophosphate and AMP involved in the synthesis of ATP and GTP, prompting us to investigate ADS lyase in <i>C. neoformans.</i> Here, we report that <i>ADE13</i> encodes ADS lyase in <i>C. neoformans.</i> We found that an <i>ade13</i>Δ mutant is an adenine auxotroph and is unable to successfully cause infection","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jul","modification":"2026-05-05T10:47:55.254Z","creation":"2019-03-26T23:46:09Z"},"accession":"S-EPMC5512076","cross_references":{"pubmed":["28559277"],"doi":["10.1074/jbc.M117.787994","10.1074/jbc.m117.787994"]}}