biostudies-literatureChitty JLNational Health and Medical Research Council11829-11839https://www.ebi.ac.uk/biostudies/studies/S-EPMC5512076biostudies-literatureUnknown292(28)There is significant clinical need for new antifungal agents to manage infections with pathogenic species such as <i>Cryptococcus neoformans</i> Because the purine biosynthesis pathway is essential for many metabolic processes, such as synthesis of DNA and RNA and energy generation, it may represent a potential target for developing new antifungals. Within this pathway, the bifunctional enzyme adenylosuccinate (ADS) lyase plays a role in the formation of the key intermediates inosine monophosphate and AMP involved in the synthesis of ATP and GTP, prompting us to investigate ADS lyase in <i>C. neoformans.</i> Here, we report that <i>ADE13</i> encodes ADS lyase in <i>C. neoformans.</i> We found that an <i>ade13</i>Δ mutant is an adenine auxotroph and is unable to successfully cause infectionThe Journal of biological chemistry<i>Cryptococcus neoformans</i> ADS lyase is an enzyme essential for virulence whose crystal structure reveals features exploitable in antifungal drug design.PMC5512076APP10497161003325, 1110971Chitty JLKappler UButler MSFraser JABlake KLThompson MKoh YQAERobertson AABWilliams SJBlundell RDCooper MAKobe Bfalse<i>Cryptococcus neoformans</i> ADS lyase is an enzyme essential for virulence whose crystal structure reveals features exploitable in antifungal drug design.There is significant clinical need for new antifungal agents to manage infections with pathogenic species such as <i>Cryptococcus neoformans</i> Because the purine biosynthesis pathway is essential for many metabolic processes, such as synthesis of DNA and RNA and energy generation, it may represent a potential target for developing new antifungals. Within this pathway, the bifunctional enzyme adenylosuccinate (ADS) lyase plays a role in the formation of the key intermediates inosine monophosphate and AMP involved in the synthesis of ATP and GTP, prompting us to investigate ADS lyase in <i>C. neoformans.</i> Here, we report that <i>ADE13</i> encodes ADS lyase in <i>C. neoformans.</i> We found that an <i>ade13</i>Δ mutant is an adenine auxotroph and is unable to successfully cause infection2017-01-01T00:00:00Z2017 Jul2026-05-05T10:47:55.254Z2019-03-26T23:46:09ZS-EPMC55120762855927710.1074/jbc.M117.78799410.1074/jbc.m117.787994