{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chung JO"],"funding":["NICHD NIH HHS","NIDDK NIH HHS","NCRR NIH HHS","U.S. Public Health Service"],"pagination":["2082-2091"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5521869"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["66(8)"],"pubmed_abstract":["We investigated the relationship between insulin resistance markers and subsarcolemmal (SS) and intramyofibrillar (IMF) ceramide concentrations, as well as the contribution of plasma palmitate (6.5-h infusion of [U-<sup>13</sup>C]palmitate) to intramyocellular ceramides. Seventy-six postabsorptive men and women had muscle biopsies 1.5, 6.5, and 24 h after starting the tracer infusion. Concentrations and enrichment of muscle ceramides were measured by liquid chromatography-tandem mass spectrometry. We found that HOMA of insulin resistance, plasma insulin, and triglyceride concentrations were positively correlated with SS C16:0 and C18:1 ceramide, but not SS C14:0-Cer, C20:0-Cer, C24:0-Cer, and C24:1-Cer concentrations; IMF ceramide concentrations were not correlated with any metabolic param"],"journal":["Diabetes"],"pubmed_title":["Intramyocellular Ceramides: Subcellular Concentrations and Fractional De Novo Synthesis in Postabsorptive Humans."],"pmcid":["PMC5521869"],"funding_grant_id":["R-00585","R01 DK045343","T32 DK007352","P30 DK050456","K12 HD065987","DK-45343","DK-50456","M01 RR000585","R01 DK040484","R37 DK040484","DK-40484"],"pubmed_authors":["Hames KC","Blachnio-Zabielska AU","Jensen MD","Chung JO","Koutsari C"],"additional_accession":[]},"is_claimable":false,"name":"Intramyocellular Ceramides: Subcellular Concentrations and Fractional De Novo Synthesis in Postabsorptive Humans.","description":"We investigated the relationship between insulin resistance markers and subsarcolemmal (SS) and intramyofibrillar (IMF) ceramide concentrations, as well as the contribution of plasma palmitate (6.5-h infusion of [U-<sup>13</sup>C]palmitate) to intramyocellular ceramides. Seventy-six postabsorptive men and women had muscle biopsies 1.5, 6.5, and 24 h after starting the tracer infusion. Concentrations and enrichment of muscle ceramides were measured by liquid chromatography-tandem mass spectrometry. We found that HOMA of insulin resistance, plasma insulin, and triglyceride concentrations were positively correlated with SS C16:0 and C18:1 ceramide, but not SS C14:0-Cer, C20:0-Cer, C24:0-Cer, and C24:1-Cer concentrations; IMF ceramide concentrations were not correlated with any metabolic param","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Aug","modification":"2025-04-04T20:54:32.965Z","creation":"2019-03-26T23:48:32Z"},"accession":"S-EPMC5521869","cross_references":{"pubmed":["28483801"],"doi":["10.2337/db17-0082"]}}