{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tsai FJ"],"funding":["Ministry of Science and Technology","Clinical Trial Center, China Medical University Hospital (TW)","Taiwan Ministry of Health and Welfare Clinical Trial and Research Center of Excellence"],"pagination":["e0181423"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5524319"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(7)"],"pubmed_abstract":["In nanoflow liquid chromatography-matrix-assisted laser desorption/ionization tandem time-of-flight (nanoLC-MALDI-TOF/TOF) approaches, it is critical to directly apply small amounts of the sample elutes on the sample target using a nanoLC system due to its low flow rate of 200 ~ 300 nl/min. It is recommended to apply a sheath liquid containing a matrix with a several μL/min flow rate at the end of the nanoLC column to ensure a larger co-eluted droplet for more reproducible sample spotting and avoid the laborious task of post-manual matrix spotting. In this study, to achieve a better nanoLC-MALDI performance on sample spotting, we first compared α-Cyano-4-hydroxycinnamic acid (CHCA) solvent composition for efficiently concentrating nanoLC elutes on an anchor chip. The solvent composition of"],"journal":["PloS one"],"pubmed_title":["The comparison of CHCA solvent compositions for improving LC-MALDI performance and its application to study the impact of aflatoxin B1 on the liver proteome of diabetes mellitus type 1 mice."],"pmcid":["PMC5524319"],"funding_grant_id":["MOST 103-2113-M-039-001-MY2","MOHW105-TDU-B-212-133019","DMR-105-090"],"pubmed_authors":["Tsai FJ","Chen CJ","Chen SY","Liao HY","Liu YC"],"additional_accession":[]},"is_claimable":false,"name":"The comparison of CHCA solvent compositions for improving LC-MALDI performance and its application to study the impact of aflatoxin B1 on the liver proteome of diabetes mellitus type 1 mice.","description":"In nanoflow liquid chromatography-matrix-assisted laser desorption/ionization tandem time-of-flight (nanoLC-MALDI-TOF/TOF) approaches, it is critical to directly apply small amounts of the sample elutes on the sample target using a nanoLC system due to its low flow rate of 200 ~ 300 nl/min. It is recommended to apply a sheath liquid containing a matrix with a several μL/min flow rate at the end of the nanoLC column to ensure a larger co-eluted droplet for more reproducible sample spotting and avoid the laborious task of post-manual matrix spotting. In this study, to achieve a better nanoLC-MALDI performance on sample spotting, we first compared α-Cyano-4-hydroxycinnamic acid (CHCA) solvent composition for efficiently concentrating nanoLC elutes on an anchor chip. The solvent composition of","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2025-06-01T01:37:34.175Z","creation":"2025-06-01T01:37:34.175Z"},"accession":"S-EPMC5524319","cross_references":{"pubmed":["28738076"],"doi":["10.1371/journal.pone.0181423"]}}