{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(7)"],"submitter":["Ihle MA"],"pubmed_abstract":["Aberrantly expressed microRNAs (miRNAs) are involved in many diseases including cancer. In gastrointestinal stromal tumours (GISTs) expression of miR-221 and miR-222 is reduced compared to control tissue and other sarcomas but the functional effects of this downregulation are not fully understood. This study aimed at evaluating the miR-221 and miR-222 expression profiles in different GIST subtypes and the functional role of these miRNAs. Expression of miR-221 and miR-222 was analysed in six KIT exon 9 and three KIT exon 11 mutated and nine wildtype GISTs by qPCR. Viability and apoptosis were examined in three different, KIT positive GIST cell lines (GIST882, GIST-T1 and GIST48) after overexpression of these miRNAs. The modulation of KIT and the PI3K/AKT pathways was determined by Western b"],"journal":["Molecular oncology"],"pagination":["1421-33"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5528808"],"repository":["biostudies-literature"],"pubmed_title":["miRNA-221 and miRNA-222 induce apoptosis via the KIT/AKT signalling pathway in gastrointestinal stromal tumours."],"pmcid":["PMC5528808"],"pubmed_authors":["Ihle MA","Huss S","Schildhaus HU","Buettner R","Wardelmann E","Fassunke J","Trautmann M","Bauer S","Merkelbach-Bruse S","Kuenstlinger H","Heydt C"],"additional_accession":[]},"is_claimable":false,"name":"miRNA-221 and miRNA-222 induce apoptosis via the KIT/AKT signalling pathway in gastrointestinal stromal tumours.","description":"Aberrantly expressed microRNAs (miRNAs) are involved in many diseases including cancer. In gastrointestinal stromal tumours (GISTs) expression of miR-221 and miR-222 is reduced compared to control tissue and other sarcomas but the functional effects of this downregulation are not fully understood. This study aimed at evaluating the miR-221 and miR-222 expression profiles in different GIST subtypes and the functional role of these miRNAs. Expression of miR-221 and miR-222 was analysed in six KIT exon 9 and three KIT exon 11 mutated and nine wildtype GISTs by qPCR. Viability and apoptosis were examined in three different, KIT positive GIST cell lines (GIST882, GIST-T1 and GIST48) after overexpression of these miRNAs. The modulation of KIT and the PI3K/AKT pathways was determined by Western b","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Aug","modification":"2025-04-26T08:54:50.546Z","creation":"2019-03-27T02:51:44Z"},"accession":"S-EPMC5528808","cross_references":{"pubmed":["25898773"],"doi":["10.1016/j.molonc.2015.03.013"]}}