biostudies-literatureStrauch BMDeutsche ForschungsgemeinschaftLandesgraduiertenförderung Baden-Württemberg28https://www.ebi.ac.uk/biostudies/studies/S-EPMC5540434biostudies-literatureUnknown14(1)<h4>Background</h4>Nano- and microscale copper oxide particles (CuO NP, CuO MP) are applied for manifold purposes, enhancing exposure and thus the potential risk of adverse health effects. Based on the pronounced in vitro cytotoxicity of CuO NP, systematic investigations on the mode of action are required. Therefore, the impact of CuO NP, CuO MP and CuCl₂ on the DNA damage response on transcriptional level was investigated by quantitative gene expression profiling via high-throughput RT-qPCR. Cytotoxicity, copper uptake and the impact on the oxidative stress response, cell cycle regulation and apoptosis were further analysed on the functional level.<h4>Results</h4>Cytotoxicity of CuO NP was more pronounced when compared to CuO MP and CuCl₂ in human bronchial epitheliaParticle and fibre toxicologyComparison between micro- and nanosized copper oxide and water soluble copper chloride: interrelationship between intracellular copper concentrations, oxidative stress and DNA damage response in human lung cells.PMC5540434INST 121384/24-1 FUGGNiemand RKHartwig AStrauch BMWinkelbeiner NLfalseComparison between micro- and nanosized copper oxide and water soluble copper chloride: interrelationship between intracellular copper concentrations, oxidative stress and DNA damage response in human lung cells.<h4>Background</h4>Nano- and microscale copper oxide particles (CuO NP, CuO MP) are applied for manifold purposes, enhancing exposure and thus the potential risk of adverse health effects. Based on the pronounced in vitro cytotoxicity of CuO NP, systematic investigations on the mode of action are required. Therefore, the impact of CuO NP, CuO MP and CuCl₂ on the DNA damage response on transcriptional level was investigated by quantitative gene expression profiling via high-throughput RT-qPCR. Cytotoxicity, copper uptake and the impact on the oxidative stress response, cell cycle regulation and apoptosis were further analysed on the functional level.<h4>Results</h4>Cytotoxicity of CuO NP was more pronounced when compared to CuO MP and CuCl₂ in human bronchial epithelia2017-01-01T00:00:00Z2017 Aug2025-05-31T23:31:51.204Z2025-05-31T23:31:51.204ZS-EPMC55404342876471510.1186/s12989-017-0209-1