{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(1)"],"submitter":["Gajer M"],"pubmed_abstract":["Hepadnaviruses, including human hepatitis B virus (HBV), replicate their tiny DNA genomes by protein-primed reverse transcription of a pregenomic (pg) RNA. Replication initiation as well as pgRNA encapsidation depend on the interaction of the viral polymerase, P protein, with the ε RNA element, featuring a lower and an upper stem, a central bulge, and an apical loop. The bulge, somehow assisted by the loop, acts as template for a P protein-linked DNA oligo that primes full-length minus-strand DNA synthesis. Phylogenetic conservation and earlier mutational studies suggested the highly based-paired ε structure as crucial for productive interaction with P protein. Using the tractable duck HBV (DHBV) model we here interrogated the entire apical DHBV ε (Dε) half for sequence- and structure-depe"],"journal":["Scientific reports"],"pagination":["7120"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5541001"],"repository":["biostudies-literature"],"pubmed_title":["Few basepairing-independent motifs in the apical half of the avian HBV ε RNA stem-loop determine site-specific initiation of protein-priming."],"pmcid":["PMC5541001"],"pubmed_authors":["Schmid B","Rosler C","Beck J","Nassal M","Gajer M","Dornbrack K"],"additional_accession":[]},"is_claimable":false,"name":"Few basepairing-independent motifs in the apical half of the avian HBV ε RNA stem-loop determine site-specific initiation of protein-priming.","description":"Hepadnaviruses, including human hepatitis B virus (HBV), replicate their tiny DNA genomes by protein-primed reverse transcription of a pregenomic (pg) RNA. Replication initiation as well as pgRNA encapsidation depend on the interaction of the viral polymerase, P protein, with the ε RNA element, featuring a lower and an upper stem, a central bulge, and an apical loop. The bulge, somehow assisted by the loop, acts as template for a P protein-linked DNA oligo that primes full-length minus-strand DNA synthesis. Phylogenetic conservation and earlier mutational studies suggested the highly based-paired ε structure as crucial for productive interaction with P protein. Using the tractable duck HBV (DHBV) model we here interrogated the entire apical DHBV ε (Dε) half for sequence- and structure-depe","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Aug","modification":"2026-04-13T13:35:53.021Z","creation":"2019-03-27T02:52:29Z"},"accession":"S-EPMC5541001","cross_references":{"pubmed":["28769080"],"doi":["10.1038/s41598-017-07657-z"]}}