biostudies-literatureFantuzzo JANIDA NIH HHSNIAAA NIH HHS87-97https://www.ebi.ac.uk/biostudies/studies/S-EPMC5541685biostudies-literatureUnknown5(2)Neurocircuits in the human brain govern complex behavior and involve connections from many different neuronal subtypes from different brain regions. Recent advances in stem cell biology have enabled the derivation of patient-specific human neuronal cells of various subtypes for the study of neuronal function and disease pathology. Nevertheless, one persistent challenge using these human-derived neurons is the ability to reconstruct models of human brain circuitry. To overcome this obstacle, we have developed a compartmentalized microfluidic device, which allows for spatial separation of cell bodies of different human-derived neuronal subtypes (excitatory, inhibitory and dopaminergic) but is permissive to the spreading of projecting processes. Induced neurons (iNs) cultured in the device exTechnologyμNeurocircuitry: Establishing <i>in vitro</i> models of neurocircuits with human neurons.PMC5541685R21 DA032984R01 AA023797R21 DA035594R21 DA039686Hart RPFantuzzo JALiu JJMcGowan HYang NWernig MHalikere APang ZPNg YHDe Filippis LZahn JDfalseμNeurocircuitry: Establishing <i>in vitro</i> models of neurocircuits with human neurons.Neurocircuits in the human brain govern complex behavior and involve connections from many different neuronal subtypes from different brain regions. Recent advances in stem cell biology have enabled the derivation of patient-specific human neuronal cells of various subtypes for the study of neuronal function and disease pathology. Nevertheless, one persistent challenge using these human-derived neurons is the ability to reconstruct models of human brain circuitry. To overcome this obstacle, we have developed a compartmentalized microfluidic device, which allows for spatial separation of cell bodies of different human-derived neuronal subtypes (excitatory, inhibitory and dopaminergic) but is permissive to the spreading of projecting processes. Induced neurons (iNs) cultured in the device ex2017-01-01T00:00:00Z2017 Jun2025-04-04T02:58:04.182Z2019-03-26T23:38:56ZS-EPMC55416852878199310.1142/S233954781750005410.1142/s2339547817500054