{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(1)"],"submitter":["van Eijl RAPM"],"pubmed_abstract":["In this study, we originally aimed to characterize the potential role of Argonaute 2 (AGO2) in the nucleus, a key protein of the miRNA machinery. We combined Chromatin Immunoprecipitation (ChIP) with high throughput sequencing (ChIP-seq) and quantitative mass spectrometry (ChIP-MS) using the broadly used AGO2 11A9 antibody to determine interactions with chromatin and nuclear proteins. We found a previously described interaction between AGO2 and SWI/SNF on chromatin with ChIP-MS and observed enrichment at enhancers and transcription start sites using ChIP-seq. However, antibody specificity issues can produce misleading results for ChIP, RNA-seq and Mass spectrometry. Therefore, we developed a CRISPR/Cas9 engineered AGO2<sup>-/-</sup> HEK293T cell line to validate our findings. ChIP-qPCR and"],"journal":["Scientific reports"],"pagination":["7278"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5544689"],"repository":["biostudies-literature"],"pubmed_title":["Reactivity of human AGO2 monoclonal antibody 11A9 with the SWI/SNF complex: A case study for rigorously defining antibody selectivity."],"pmcid":["PMC5544689"],"pubmed_authors":["van den Brand T","Nguyen LN","van Eijl RAPM","Mulder KW"],"additional_accession":[]},"is_claimable":false,"name":"Reactivity of human AGO2 monoclonal antibody 11A9 with the SWI/SNF complex: A case study for rigorously defining antibody selectivity.","description":"In this study, we originally aimed to characterize the potential role of Argonaute 2 (AGO2) in the nucleus, a key protein of the miRNA machinery. We combined Chromatin Immunoprecipitation (ChIP) with high throughput sequencing (ChIP-seq) and quantitative mass spectrometry (ChIP-MS) using the broadly used AGO2 11A9 antibody to determine interactions with chromatin and nuclear proteins. We found a previously described interaction between AGO2 and SWI/SNF on chromatin with ChIP-MS and observed enrichment at enhancers and transcription start sites using ChIP-seq. However, antibody specificity issues can produce misleading results for ChIP, RNA-seq and Mass spectrometry. Therefore, we developed a CRISPR/Cas9 engineered AGO2<sup>-/-</sup> HEK293T cell line to validate our findings. ChIP-qPCR and","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Aug","modification":"2025-04-26T12:30:49.111Z","creation":"2019-03-27T02:52:45Z"},"accession":"S-EPMC5544689","cross_references":{"pubmed":["28779093"],"doi":["10.1038/s41598-017-07539-4"]}}