{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Drozdz MM"],"funding":["Medical Research Council","Biotechnology and Biological Sciences Research Council"],"pagination":["7454"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5547041"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(1)"],"pubmed_abstract":["Structure of interphase cell nuclei remains dynamic and can undergo various changes of shape and organisation, in health and disease. The double-membraned envelope that separates nuclear genetic material from the rest of the cell frequently includes deep, branching tubular invaginations that form a dynamic nucleoplasmic reticulum (NR). This study addresses mechanisms by which NR can form in interphase nuclei. We present a combination of Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS) approach and light microscopy techniques to follow formation of NR by using pulse-chase experiments to examine protein and lipid delivery to nascent NR in cultured cells. Lamina protein incorporation was assessed using precursor accumulation (for lamin A) or a MAPLE3 photoconvertible tag (for lamin B1) an"],"journal":["Scientific reports"],"pubmed_title":["Formation of a nucleoplasmic reticulum requires de novo assembly of nascent phospholipids and shows preferential incorporation of nascent lamins."],"pmcid":["PMC5547041"],"funding_grant_id":["BB/L002159/1","G0801917"],"pubmed_authors":["Grovenor C","Pytowski L","Jiang H","Vaux DJ","Drozdz MM"],"additional_accession":[]},"is_claimable":false,"name":"Formation of a nucleoplasmic reticulum requires de novo assembly of nascent phospholipids and shows preferential incorporation of nascent lamins.","description":"Structure of interphase cell nuclei remains dynamic and can undergo various changes of shape and organisation, in health and disease. The double-membraned envelope that separates nuclear genetic material from the rest of the cell frequently includes deep, branching tubular invaginations that form a dynamic nucleoplasmic reticulum (NR). This study addresses mechanisms by which NR can form in interphase nuclei. We present a combination of Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS) approach and light microscopy techniques to follow formation of NR by using pulse-chase experiments to examine protein and lipid delivery to nascent NR in cultured cells. Lamina protein incorporation was assessed using precursor accumulation (for lamin A) or a MAPLE3 photoconvertible tag (for lamin B1) an","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Aug","modification":"2026-07-15T15:44:51.181Z","creation":"2019-03-27T02:52:53Z"},"accession":"S-EPMC5547041","cross_references":{"pubmed":["28785031"],"doi":["10.1038/s41598-017-07614-w"]}}