{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Vergne I"],"funding":["NIAID NIH HHS"],"pagination":["4033-8"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC554822"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["102(11)"],"pubmed_abstract":["Live Mycobacterium tuberculosis persists in macrophage phagosomes by interfering with phagolysosome biogenesis. Here, using four-dimensional microscopy and in vitro assays, we report the principal difference between phagosomes containing live and dead mycobacteria. Phosphatidylinositol 3-phosphate (PI3P), a membrane trafficking regulatory lipid essential for phagosomal acquisition of lysosomal constituents, is retained on phagosomes harboring dead mycobacteria but is continuously eliminated from phagosomes with live bacilli. We show that the exclusion of PI3P from live mycobacterial phagosomes can be only transiently reversed by Ca2+ fluxes, and that live M. tuberculosis secretes a lipid phosphatase, SapM, that hydrolyzes PI3P, inhibits phagosome-late endosome fusion in vitro, and contribu"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Mechanism of phagolysosome biogenesis block by viable Mycobacterium tuberculosis."],"pmcid":["PMC554822"],"funding_grant_id":["AI45148","N01 AI-75320","R01 AI045148"],"pubmed_authors":["Vergne I","Belisle J","Deretic V","Lucas M","Lee HH","Chua J"],"additional_accession":[]},"is_claimable":false,"name":"Mechanism of phagolysosome biogenesis block by viable Mycobacterium tuberculosis.","description":"Live Mycobacterium tuberculosis persists in macrophage phagosomes by interfering with phagolysosome biogenesis. Here, using four-dimensional microscopy and in vitro assays, we report the principal difference between phagosomes containing live and dead mycobacteria. Phosphatidylinositol 3-phosphate (PI3P), a membrane trafficking regulatory lipid essential for phagosomal acquisition of lysosomal constituents, is retained on phagosomes harboring dead mycobacteria but is continuously eliminated from phagosomes with live bacilli. We show that the exclusion of PI3P from live mycobacterial phagosomes can be only transiently reversed by Ca2+ fluxes, and that live M. tuberculosis secretes a lipid phosphatase, SapM, that hydrolyzes PI3P, inhibits phagosome-late endosome fusion in vitro, and contribu","dates":{"release":"2005-01-01T00:00:00Z","publication":"2005 Mar","modification":"2026-05-06T06:31:20.794Z","creation":"2025-06-01T00:31:45.142Z"},"accession":"S-EPMC554822","cross_references":{"pubmed":["15753315"],"doi":["10.1073/pnas.0409716102"]}}