{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(1)"],"submitter":["Jung D"],"pubmed_abstract":["We present a portable multi-contrast microscope capable of producing bright-field, dark-field, and differential phase contrast images of thin biological specimens on a smartphone platform. The microscopy method is based on an imaging scheme termed \"color-coded light-emitting-diode (LED) microscopy (cLEDscope),\" in which a specimen is illuminated with a color-coded LED array and light transmitted through the specimen is recorded by a color image sensor. Decomposition of the image into red, green, and blue colors and subsequent computation enable multi-contrast imaging in a single shot. In order to transform a smartphone into a multi-contrast imaging device, we developed an add-on module composed of a patterned color micro-LED array, specimen stage, and miniature objective. Simple installation of this module onto a smartphone enables multi-contrast imaging of transparent specimens. In addition, an Android-based app was implemented to acquire an image, perform the associated computation, and display the multi-contrast images in real time. Herein, the details of our smartphone module and experimental demonstrations with various biological specimens are presented."],"journal":["Scientific reports"],"pagination":["7564"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5548908"],"repository":["biostudies-literature"],"pubmed_title":["Smartphone-based multi-contrast microscope using color-multiplexed illumination."],"pmcid":["PMC5548908"],"pubmed_authors":["Kim S","Lee JS","Jung D","Choi JH","Joo C","Ryu S","Lee W"],"additional_accession":[]},"is_claimable":false,"name":"Smartphone-based multi-contrast microscope using color-multiplexed illumination.","description":"We present a portable multi-contrast microscope capable of producing bright-field, dark-field, and differential phase contrast images of thin biological specimens on a smartphone platform. The microscopy method is based on an imaging scheme termed \"color-coded light-emitting-diode (LED) microscopy (cLEDscope),\" in which a specimen is illuminated with a color-coded LED array and light transmitted through the specimen is recorded by a color image sensor. Decomposition of the image into red, green, and blue colors and subsequent computation enable multi-contrast imaging in a single shot. In order to transform a smartphone into a multi-contrast imaging device, we developed an add-on module composed of a patterned color micro-LED array, specimen stage, and miniature objective. Simple installation of this module onto a smartphone enables multi-contrast imaging of transparent specimens. In addition, an Android-based app was implemented to acquire an image, perform the associated computation, and display the multi-contrast images in real time. Herein, the details of our smartphone module and experimental demonstrations with various biological specimens are presented.","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Aug","modification":"2025-05-29T19:17:25.69Z","creation":"2025-05-29T19:17:25.69Z"},"accession":"S-EPMC5548908","cross_references":{"pubmed":["28790342"],"doi":["10.1038/s41598-017-07703-w"]}}