{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(7)"],"submitter":["Delbridge AR"],"pubmed_abstract":["Anaemia is a major global health problem arising from diverse causes and for which improved therapeutic strategies are needed. Erythroid cells can undergo apoptotic cell death and loss of pro-survival BCL-XL is known to trigger apoptosis during late-stage erythroid development. However, the mechanism by which loss or pharmacological blockade of BCL-XL leads to erythroid cell apoptosis remains unclear. Here we sought to identify the precise stage of erythropoiesis that depends on BCL-XL. We also tested whether deficiency of BIM or PUMA, the two main pro-apoptotic antagonists of BCL-XL, could prevent reticulocyte death and anaemia caused by BCL-XL loss. Using an in vivo mouse model of tamoxifen-inducible Bclx gene deletion and in vitro assays with a BCL-XL-selective inhibitor, we interrogate"],"journal":["Cell death & disease"],"pagination":["e2914"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5550852"],"repository":["biostudies-literature"],"pubmed_title":["The BH3-only proteins BIM and PUMA are not critical for the reticulocyte apoptosis caused by loss of the pro-survival protein BCL-XL."],"pmcid":["PMC5550852"],"pubmed_authors":["Hyland C","Garnier JM","Bernardini JP","Delbridge AR","Aubrey BJ","Di Rago L","Strasser A","Alexander WS","Lessene G","Grabow S"],"additional_accession":[]},"is_claimable":false,"name":"The BH3-only proteins BIM and PUMA are not critical for the reticulocyte apoptosis caused by loss of the pro-survival protein BCL-XL.","description":"Anaemia is a major global health problem arising from diverse causes and for which improved therapeutic strategies are needed. Erythroid cells can undergo apoptotic cell death and loss of pro-survival BCL-XL is known to trigger apoptosis during late-stage erythroid development. However, the mechanism by which loss or pharmacological blockade of BCL-XL leads to erythroid cell apoptosis remains unclear. Here we sought to identify the precise stage of erythropoiesis that depends on BCL-XL. We also tested whether deficiency of BIM or PUMA, the two main pro-apoptotic antagonists of BCL-XL, could prevent reticulocyte death and anaemia caused by BCL-XL loss. Using an in vivo mouse model of tamoxifen-inducible Bclx gene deletion and in vitro assays with a BCL-XL-selective inhibitor, we interrogate","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jul","modification":"2025-04-05T14:18:51.62Z","creation":"2019-03-27T02:53:08Z"},"accession":"S-EPMC5550852","cross_references":{"pubmed":["28682312"],"doi":["10.1038/cddis.2017.304"]}}