{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Marvin RK"],"funding":["NIDCR NIH HHS","National Institute of Dental and Craniofacial Research","Air Force Office of Scientific Research","National Science Foundation"],"pagination":["372-382"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5551674"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(3-4)"],"pubmed_abstract":["<h4>Context</h4>Quantitative changes of salivary proteins due to acute stress were detected.<h4>Objective</h4>To explore protein markers of stress in saliva of eight medical residents who performed emergency medicine simulations.<h4>Materials and methods</h4>Saliva was collected before the simulations, after the simulations, and following morning upon waking. Proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), identified by mass spectrometry (MS), and relatively quantified by densitometry.<h4>Results</h4>Salivary alpha-amylase and S-type cystatins significantly increased, while the ∼26 kDa and low-molecular weight (MW) (<10 kDa) SDS-PAGE bands exhibited changes after stress.<h4>Discussion and conclusion</h4>Alpha-amylase and cystatins are poten"],"journal":["Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals"],"pubmed_title":["Salivary protein changes in response to acute stress in medical residents performing advanced clinical simulations: a pilot proteomics study."],"pmcid":["PMC5551674"],"funding_grant_id":["0923184","DE023418","R21 DE023418","18","84"],"pubmed_authors":["Rega P","Ye S","Isailovic D","Giovannucci DR","Saepoo MB","White DB","Kazan V","Liu R","Hensley K","Marvin RK"],"additional_accession":[]},"is_claimable":false,"name":"Salivary protein changes in response to acute stress in medical residents performing advanced clinical simulations: a pilot proteomics study.","description":"<h4>Context</h4>Quantitative changes of salivary proteins due to acute stress were detected.<h4>Objective</h4>To explore protein markers of stress in saliva of eight medical residents who performed emergency medicine simulations.<h4>Materials and methods</h4>Saliva was collected before the simulations, after the simulations, and following morning upon waking. Proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), identified by mass spectrometry (MS), and relatively quantified by densitometry.<h4>Results</h4>Salivary alpha-amylase and S-type cystatins significantly increased, while the ∼26 kDa and low-molecular weight (MW) (<10 kDa) SDS-PAGE bands exhibited changes after stress.<h4>Discussion and conclusion</h4>Alpha-amylase and cystatins are poten","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 May - Jun","modification":"2026-04-15T17:24:50.816Z","creation":"2019-03-26T23:34:07Z"},"accession":"S-EPMC5551674","cross_references":{"pubmed":["28055279"],"doi":["10.1080/1354750X.2017.1279215","10.1080/1354750x.2017.1279215"]}}