biostudies-literatureMarvin RKNIDCR NIH HHSNational Institute of Dental and Craniofacial ResearchAir Force Office of Scientific ResearchNational Science Foundation372-382https://www.ebi.ac.uk/biostudies/studies/S-EPMC5551674biostudies-literatureUnknown22(3-4)<h4>Context</h4>Quantitative changes of salivary proteins due to acute stress were detected.<h4>Objective</h4>To explore protein markers of stress in saliva of eight medical residents who performed emergency medicine simulations.<h4>Materials and methods</h4>Saliva was collected before the simulations, after the simulations, and following morning upon waking. Proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), identified by mass spectrometry (MS), and relatively quantified by densitometry.<h4>Results</h4>Salivary alpha-amylase and S-type cystatins significantly increased, while the ∼26 kDa and low-molecular weight (MW) (<10 kDa) SDS-PAGE bands exhibited changes after stress.<h4>Discussion and conclusion</h4>Alpha-amylase and cystatins are potenBiomarkers : biochemical indicators of exposure, response, and susceptibility to chemicalsSalivary protein changes in response to acute stress in medical residents performing advanced clinical simulations: a pilot proteomics study.PMC55516740923184DE023418R21 DE0234181884Rega PYe SIsailovic DGiovannucci DRSaepoo MBWhite DBKazan VLiu RHensley KMarvin RKfalseSalivary protein changes in response to acute stress in medical residents performing advanced clinical simulations: a pilot proteomics study.<h4>Context</h4>Quantitative changes of salivary proteins due to acute stress were detected.<h4>Objective</h4>To explore protein markers of stress in saliva of eight medical residents who performed emergency medicine simulations.<h4>Materials and methods</h4>Saliva was collected before the simulations, after the simulations, and following morning upon waking. Proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), identified by mass spectrometry (MS), and relatively quantified by densitometry.<h4>Results</h4>Salivary alpha-amylase and S-type cystatins significantly increased, while the ∼26 kDa and low-molecular weight (MW) (<10 kDa) SDS-PAGE bands exhibited changes after stress.<h4>Discussion and conclusion</h4>Alpha-amylase and cystatins are poten2017-01-01T00:00:00Z2017 May - Jun2026-04-15T17:24:50.816Z2019-03-26T23:34:07ZS-EPMC55516742805527910.1080/1354750X.2017.127921510.1080/1354750x.2017.1279215