{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mahanty A"],"funding":["ICAR-NFBSFARA"],"pagination":["617"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5557063"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(1)"],"pubmed_abstract":["<h4>Background</h4>High ambient temperature is known to affect fish gonadal development and physiology in a variety of ways depending on the severity and duration of exposure; however, the underlying molecular mechanisms are poorly understood. Gonadal gene expression influence the gonadal development, physiology and the quality of egg/sperm produced in teleosts and the mechanistic understanding of spatio-temporal changes in the gonadal gene expression could be instrumental in controlling the fate of egg/sperm and the quality of seed produced. Real time-quantititative polymerase chain reaction (RT-qCR), is a high throughput, sensitive and reproducible methodology used for understanding gene expression patterns by measuring the relative abundance of mRNA transcripts. However, its accuracy re"],"journal":["BMC genomics"],"pubmed_title":["Suitable reference gene for quantitative real-time PCR analysis of gene expression in gonadal tissues of minnow Puntius sophore under high-temperature stress."],"pmcid":["PMC5557063"],"funding_grant_id":["AS-2001"],"pubmed_authors":["Mohanty S","Purohit GK","Mahanty A","Nayak NR","Mohanty BP"],"additional_accession":[]},"is_claimable":false,"name":"Suitable reference gene for quantitative real-time PCR analysis of gene expression in gonadal tissues of minnow Puntius sophore under high-temperature stress.","description":"<h4>Background</h4>High ambient temperature is known to affect fish gonadal development and physiology in a variety of ways depending on the severity and duration of exposure; however, the underlying molecular mechanisms are poorly understood. Gonadal gene expression influence the gonadal development, physiology and the quality of egg/sperm produced in teleosts and the mechanistic understanding of spatio-temporal changes in the gonadal gene expression could be instrumental in controlling the fate of egg/sperm and the quality of seed produced. Real time-quantititative polymerase chain reaction (RT-qCR), is a high throughput, sensitive and reproducible methodology used for understanding gene expression patterns by measuring the relative abundance of mRNA transcripts. However, its accuracy re","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Aug","modification":"2025-04-04T08:46:26.499Z","creation":"2019-06-06T17:56:29Z"},"accession":"S-EPMC5557063","cross_references":{"pubmed":["28810828"],"doi":["10.1186/s12864-017-3974-1"]}}