biostudies-literatureUnknown24(6)Lario PIThe ability to translocate virulence proteins into host cells through a type III secretion apparatus (TTSS) is a hallmark of several Gram-negative pathogens including Shigella, Salmonella, Yersinia, Pseudomonas, and enteropathogenic Escherichia coli. In common with other types of bacterial secretion apparatus, the assembly of the TTSS complex requires the preceding formation of its integral outer membrane secretin ring component. We have determined at 1.5 A the structure of MxiM28-142, the Shigella pilot protein that is essential for the assembly and membrane association of the Shigella secretin, MxiD. This represents the first atomic structure of a secretin pilot protein from the several bacterial secretion systems containing an orthologous secretin component. A deep hydrophobic cavity isThe EMBO journal1111-21https://www.ebi.ac.uk/biostudies/studies/S-EPMC556411biostudies-literatureStructure and biochemical analysis of a secretin pilot protein.PMC556411Maurelli ATHaynes CPfuetzner RACreagh LLario PIFrey EAStrynadka NCfalseStructure and biochemical analysis of a secretin pilot protein.The ability to translocate virulence proteins into host cells through a type III secretion apparatus (TTSS) is a hallmark of several Gram-negative pathogens including Shigella, Salmonella, Yersinia, Pseudomonas, and enteropathogenic Escherichia coli. In common with other types of bacterial secretion apparatus, the assembly of the TTSS complex requires the preceding formation of its integral outer membrane secretin ring component. We have determined at 1.5 A the structure of MxiM28-142, the Shigella pilot protein that is essential for the assembly and membrane association of the Shigella secretin, MxiD. This represents the first atomic structure of a secretin pilot protein from the several bacterial secretion systems containing an orthologous secretin component. A deep hydrophobic cavity is2005-01-01T00:00:00Z2005 Mar2025-04-04T23:12:24.159Z2019-03-27T01:08:41ZS-EPMC5564111577597410.1038/sj.emboj.7600610