biostudies-literatureVandeKopple MJNIDDK NIH HHSNCI NIH HHS27-38https://www.ebi.ac.uk/biostudies/studies/S-EPMC5567683biostudies-literatureUnknown235(1)Hypoxia-inducible lipid droplet-associated protein (HILPDA) has been shown to localize to lipid droplets in nutrient-responsive cell types such as hepatocytes and adipocytes. However, its role in the control of whole-body homeostasis is not known. We sought to measure cell-intrinsic and systemic stress responses in a mouse strain harboring whole-body Hilpda deficiency. We generated a genetically engineered mouse model of whole-body HILPDA deficiency by replacing the coding <i>Hilpda</i> exon with luciferase. We subjected the knockout animals to environmental stresses and measured whole-animal metabolic and behavioral parameters. Brown adipocyte precursors were isolated and differentiated <i>in vitro</i> to quantify the impact of HILPDA ablation in lipid storage and mobilization in these ceThe Journal of endocrinologyStress-responsive HILPDA is necessary for thermoregulation during fasting.PMC5567683P30 CA016058R35 CA197713R03 CA191653K01 DK105109P01 CA067166Bal NCWu JMaurya SKStanford KIDenko NCPeriasamy MKalyanasundaram AGiaccia AJBaer LAPapandreou IVandeKopple MJfalseStress-responsive HILPDA is necessary for thermoregulation during fasting.Hypoxia-inducible lipid droplet-associated protein (HILPDA) has been shown to localize to lipid droplets in nutrient-responsive cell types such as hepatocytes and adipocytes. However, its role in the control of whole-body homeostasis is not known. We sought to measure cell-intrinsic and systemic stress responses in a mouse strain harboring whole-body Hilpda deficiency. We generated a genetically engineered mouse model of whole-body HILPDA deficiency by replacing the coding <i>Hilpda</i> exon with luciferase. We subjected the knockout animals to environmental stresses and measured whole-animal metabolic and behavioral parameters. Brown adipocyte precursors were isolated and differentiated <i>in vitro</i> to quantify the impact of HILPDA ablation in lipid storage and mobilization in these ce2017-01-01T00:00:00Z2017 Oct2025-04-25T18:29:46.009Z2019-06-06T17:58:53ZS-EPMC55676832873982210.1530/joe-17-028910.1530/JOE-17-0289