biostudies-literatureUnknown23(3)Tian SLCapsanthin/capsorubin synthase (<i>Ccs</i>) gene is a key gene that regulates the synthesis of capsanthin and the development of red coloration in pepper fruits. There are three tandem repeat units in the promoter region of <i>Ccs</i>, but the potential effects of the number of repetitive units on the transcriptional regulation of <i>Ccs</i> has been unclear. In the present study, expression vectors carrying different numbers of repeat units of the <i>Ccs</i> promoter were constructed, and the transient expression of the β-glucuronidase (<i>GUS</i>) gene was used to detect differences in expression levels associated with the promoter fragments. These repeat fragments and the plant expression vector PBI121 containing the 35s CaMV promoter were ligated to form recombinant vectors that were tPhysiology and molecular biology of plants : an international journal of functional plant biology685-691https://www.ebi.ac.uk/biostudies/studies/S-EPMC5567698biostudies-literatureAnalysis of tandem repeat units of the promoter of capsanthin/capsorubin synthase (<i>Ccs</i>) gene in pepper fruit.PMC5567698Li ZTian SLShah SNMGong ZHLi LfalseAnalysis of tandem repeat units of the promoter of capsanthin/capsorubin synthase (<i>Ccs</i>) gene in pepper fruit.Capsanthin/capsorubin synthase (<i>Ccs</i>) gene is a key gene that regulates the synthesis of capsanthin and the development of red coloration in pepper fruits. There are three tandem repeat units in the promoter region of <i>Ccs</i>, but the potential effects of the number of repetitive units on the transcriptional regulation of <i>Ccs</i> has been unclear. In the present study, expression vectors carrying different numbers of repeat units of the <i>Ccs</i> promoter were constructed, and the transient expression of the β-glucuronidase (<i>GUS</i>) gene was used to detect differences in expression levels associated with the promoter fragments. These repeat fragments and the plant expression vector PBI121 containing the 35s CaMV promoter were ligated to form recombinant vectors that were t2017-01-01T00:00:00Z2017 Jul2026-05-02T05:40:36.562Z2019-03-26T23:44:06ZS-EPMC55676982887850610.1007/s12298-017-0437-8