{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Patel S"],"funding":["NSF","NCCDPHP CDC HHS","NIDCD NIH HHS","NINDS NIH HHS","New Jersey Commission on Spinal Cord","NIH"],"pagination":["11983-8"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5568028"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["54(41)"],"pubmed_abstract":["Even though gene repression is a powerful approach to exogenously regulate cellular behavior, developing a platform to effectively repress targeted genes, especially for stem-cell applications, remains elusive. Herein, we introduce a nanomaterial-based platform that is capable of mimicking the function of transcription repressor proteins to downregulate gene expression at the transcriptional level for enhancing stem-cell differentiation. We developed the \"NanoScript\" platform by integrating multiple gene repression molecules with a nanoparticle. First, we show a proof-of-concept demonstration using a GFP-specific NanoScript to knockdown GFP expression in neural stem cells (NSCs-GFP). Then, we show that a Sox9-specific NanoScript can repress Sox9 expression to initiate enhanced differentiat"],"journal":["Angewandte Chemie (International ed. in English)"],"pubmed_title":["Induction of stem-cell-derived functional neurons by NanoScript-based gene repression."],"pmcid":["PMC5568028"],"funding_grant_id":["1DP20D006462-01","R01 DC015000","CHE-1429062","R21 NS085569","1R21NS085569-01","CSR13ERG005"],"pubmed_authors":["Chueng ST","Kwan K","Dardir K","Lee KB","Patel S","Sugiyama H","Pasquale N","Song Z","Yin PT"],"additional_accession":[]},"is_claimable":false,"name":"Induction of stem-cell-derived functional neurons by NanoScript-based gene repression.","description":"Even though gene repression is a powerful approach to exogenously regulate cellular behavior, developing a platform to effectively repress targeted genes, especially for stem-cell applications, remains elusive. Herein, we introduce a nanomaterial-based platform that is capable of mimicking the function of transcription repressor proteins to downregulate gene expression at the transcriptional level for enhancing stem-cell differentiation. We developed the \"NanoScript\" platform by integrating multiple gene repression molecules with a nanoparticle. First, we show a proof-of-concept demonstration using a GFP-specific NanoScript to knockdown GFP expression in neural stem cells (NSCs-GFP). Then, we show that a Sox9-specific NanoScript can repress Sox9 expression to initiate enhanced differentiat","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Oct","modification":"2025-04-21T17:48:20.272Z","creation":"2019-03-27T02:54:15Z"},"accession":"S-EPMC5568028","cross_references":{"pubmed":["26292201"],"doi":["10.1002/anie.201504902"]}}