biostudies-literaturePatel SNSFNCCDPHP CDC HHSNIDCD NIH HHSNINDS NIH HHSNew Jersey Commission on Spinal CordNIH11983-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC5568028biostudies-literatureUnknown54(41)Even though gene repression is a powerful approach to exogenously regulate cellular behavior, developing a platform to effectively repress targeted genes, especially for stem-cell applications, remains elusive. Herein, we introduce a nanomaterial-based platform that is capable of mimicking the function of transcription repressor proteins to downregulate gene expression at the transcriptional level for enhancing stem-cell differentiation. We developed the "NanoScript" platform by integrating multiple gene repression molecules with a nanoparticle. First, we show a proof-of-concept demonstration using a GFP-specific NanoScript to knockdown GFP expression in neural stem cells (NSCs-GFP). Then, we show that a Sox9-specific NanoScript can repress Sox9 expression to initiate enhanced differentiatAngewandte Chemie (International ed. in English)Induction of stem-cell-derived functional neurons by NanoScript-based gene repression.PMC55680281DP20D006462-01R01 DC015000CHE-1429062R21 NS0855691R21NS085569-01CSR13ERG005Chueng STKwan KDardir KLee KBPatel SSugiyama HPasquale NSong ZYin PTfalseInduction of stem-cell-derived functional neurons by NanoScript-based gene repression.Even though gene repression is a powerful approach to exogenously regulate cellular behavior, developing a platform to effectively repress targeted genes, especially for stem-cell applications, remains elusive. Herein, we introduce a nanomaterial-based platform that is capable of mimicking the function of transcription repressor proteins to downregulate gene expression at the transcriptional level for enhancing stem-cell differentiation. We developed the "NanoScript" platform by integrating multiple gene repression molecules with a nanoparticle. First, we show a proof-of-concept demonstration using a GFP-specific NanoScript to knockdown GFP expression in neural stem cells (NSCs-GFP). Then, we show that a Sox9-specific NanoScript can repress Sox9 expression to initiate enhanced differentiat2015-01-01T00:00:00Z2015 Oct2025-04-21T17:48:20.272Z2019-03-27T02:54:15ZS-EPMC55680282629220110.1002/anie.201504902