biostudies-literatureSgolastra FNSFNCI NIH HHSNIGMS NIH HHS131-136https://www.ebi.ac.uk/biostudies/studies/S-EPMC5568762biostudies-literatureUnknown254The impermeability of the plasma membrane towards large, hydrophilic biomolecules is a major obstacle in their use and development against intracellular targets. To overcome such limitations, protein transduction domains (PTDs) have been used as protein carriers, however they often require covalent fusion to the protein for efficient delivery. In an effort to develop more efficient and versatile biological vehicles, a series of PTD-inspired polyoxanorbornene-based synthetic mimics with identical chemical compositions but different hydrophobic/hydrophilic segregation were used to investigate the role of sequence segregation on protein binding and uptake into Jurkat T cells and HEK293Ts. This series was composed of a strongly segregated block copolymer, an intermediately segregated gradient Journal of controlled release : official journal of the Controlled Release SocietySequence segregation improves non-covalent protein delivery.PMC5568762P01 CA166009CHE-0910963T32 GM008515deRonde BMIlker Ozay EBacklund CMMinter LMSgolastra FTew GNfalseSequence segregation improves non-covalent protein delivery.The impermeability of the plasma membrane towards large, hydrophilic biomolecules is a major obstacle in their use and development against intracellular targets. To overcome such limitations, protein transduction domains (PTDs) have been used as protein carriers, however they often require covalent fusion to the protein for efficient delivery. In an effort to develop more efficient and versatile biological vehicles, a series of PTD-inspired polyoxanorbornene-based synthetic mimics with identical chemical compositions but different hydrophobic/hydrophilic segregation were used to investigate the role of sequence segregation on protein binding and uptake into Jurkat T cells and HEK293Ts. This series was composed of a strongly segregated block copolymer, an intermediately segregated gradient 2017-01-01T00:00:00Z2017 May2025-04-21T14:09:48.442Z2019-03-27T02:54:20ZS-EPMC55687622836352010.1016/j.jconrel.2017.03.387