biostudies-literatureUnknown7(1)Huang QFine particulate matter (PM2.5) exposure, especially to its organic components, induces adverse health effects on the respiratory system. However, the molecular mechanisms have still not been fully elucidated. Long non-coding RNA (lncRNA) is involved in various physio-pathological processes. In this study, the roles of lncRNA were investigated to reveal the toxicology of PM2.5. Organic extracts of PM2.5 from Nanjing and Shanghai cities were adopted to treat human bronchial epithelial cell lines (BEAS-2B and A549). RNA sequencing showed that the lncRNA functioned as antisense RNA, intergenic RNA and pre-miRNA. The mRNA profiles were also altered after exposure. PM2.5 from Nanjing showed a more serious impact than that from Shanghai. In detail, higher expression of n405968 was positively relScientific reports9392https://www.ebi.ac.uk/biostudies/studies/S-EPMC5570922biostudies-literatureFine particulate matter 2.5 exerted its toxicological effect by regulating a new layer, long non-coding RNA.PMC5570922Liu YChen JLu YDeng JHuang QChi YDong SfalseFine particulate matter 2.5 exerted its toxicological effect by regulating a new layer, long non-coding RNA.Fine particulate matter (PM2.5) exposure, especially to its organic components, induces adverse health effects on the respiratory system. However, the molecular mechanisms have still not been fully elucidated. Long non-coding RNA (lncRNA) is involved in various physio-pathological processes. In this study, the roles of lncRNA were investigated to reveal the toxicology of PM2.5. Organic extracts of PM2.5 from Nanjing and Shanghai cities were adopted to treat human bronchial epithelial cell lines (BEAS-2B and A549). RNA sequencing showed that the lncRNA functioned as antisense RNA, intergenic RNA and pre-miRNA. The mRNA profiles were also altered after exposure. PM2.5 from Nanjing showed a more serious impact than that from Shanghai. In detail, higher expression of n405968 was positively rel2017-01-01T00:00:00Z2017 Aug2026-05-01T13:28:09.608Z2019-03-27T02:54:29ZS-EPMC55709222883920310.1038/s41598-017-09818-6