biostudies-literatureUnknown7(1)Shimamura MSome synthetic and bacterial glycolipids presented by CD1d specifically activate invariant NKT (iNKT) cells bearing an invariant Vα14-Jα18 (mouse) or Vα24-Jα18 (human) TCR. The antigenic glycolipids identified to date consist of two hydrophobic chains and an α-glycoside in which the 2'-OH group is in the cis orientation toward the anomeric group, namely, either an α-galactoside or an α-glucoside. Several microbial α-mannosyl glycolipids, in which the 2'-OH group is in the trans orientation, were herein examined to establish whether they have potential to activate iNKT cells. We found that α-mannnosyl1-3 (6'-O-acyl α-mannosyl)-1-1 monoacylglycerol and cholesteryl 6'-O-acyl α-mannoside, found in Saccharopolyspora and Candida albicans, respectively, induced the activation of iNKT cells, depenScientific reports9703https://www.ebi.ac.uk/biostudies/studies/S-EPMC5574887biostudies-literatureActivation of invariant natural killer T cells stimulated with microbial α-mannosyl glycolipids.PMC5574887van den Elzen PIllarionov PYamamura MKitano NAndrew PYesilkaya HShimamura MNabeshima TfalseActivation of invariant natural killer T cells stimulated with microbial α-mannosyl glycolipids.Some synthetic and bacterial glycolipids presented by CD1d specifically activate invariant NKT (iNKT) cells bearing an invariant Vα14-Jα18 (mouse) or Vα24-Jα18 (human) TCR. The antigenic glycolipids identified to date consist of two hydrophobic chains and an α-glycoside in which the 2'-OH group is in the cis orientation toward the anomeric group, namely, either an α-galactoside or an α-glucoside. Several microbial α-mannosyl glycolipids, in which the 2'-OH group is in the trans orientation, were herein examined to establish whether they have potential to activate iNKT cells. We found that α-mannnosyl1-3 (6'-O-acyl α-mannosyl)-1-1 monoacylglycerol and cholesteryl 6'-O-acyl α-mannoside, found in Saccharopolyspora and Candida albicans, respectively, induced the activation of iNKT cells, depen2017-01-01T00:00:00Z2017 Aug2025-04-19T03:30:40.233Z2019-03-27T02:54:45ZS-EPMC55748872885217410.1038/s41598-017-10309-x