{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hollas MA"],"funding":["Engineering and Physical Sciences Research Council"],"pagination":["9449-9453"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5577508"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["56(32)"],"pubmed_abstract":["In situ monitoring of biomolecular recognition, especially at surfaces, still presents a significant technical challenge. Electron paramagnetic resonance (EPR) of biomolecules spin-labeled with nitroxides can offer uniquely sensitive and selective insights into these processes, but new spin-labeling strategies are needed. The synthesis and study of a bromoacrylaldehyde spin label (BASL), which features two attachment points with orthogonal reactivity is reported. The first examples of mannose and biotin ligands coupled to aqueous carboxy-functionalized gold nanoparticles through a spin label are presented. EPR spectra were obtained for the spin-labeled ligands both free in solution and attached to nanoparticles. The labels were recognized by the mannose-binding lectin, Con A, and the bioti"],"journal":["Angewandte Chemie (International ed. in English)"],"pubmed_title":["A Bifunctional Spin Label for Ligand Recognition on Surfaces."],"pmcid":["PMC5577508"],"funding_grant_id":["DTA","EP/N009134/1","1329960"],"pubmed_authors":["Webb SJ","Flitsch SL","Fielding AJ","Hollas MA"],"additional_accession":[]},"is_claimable":false,"name":"A Bifunctional Spin Label for Ligand Recognition on Surfaces.","description":"In situ monitoring of biomolecular recognition, especially at surfaces, still presents a significant technical challenge. Electron paramagnetic resonance (EPR) of biomolecules spin-labeled with nitroxides can offer uniquely sensitive and selective insights into these processes, but new spin-labeling strategies are needed. The synthesis and study of a bromoacrylaldehyde spin label (BASL), which features two attachment points with orthogonal reactivity is reported. The first examples of mannose and biotin ligands coupled to aqueous carboxy-functionalized gold nanoparticles through a spin label are presented. EPR spectra were obtained for the spin-labeled ligands both free in solution and attached to nanoparticles. The labels were recognized by the mannose-binding lectin, Con A, and the bioti","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Aug","modification":"2026-05-06T06:32:18.77Z","creation":"2019-03-27T02:54:59Z"},"accession":"S-EPMC5577508","cross_references":{"pubmed":["28570782"],"doi":["10.1002/anie.201703929"]}}