biostudies-literatureUnknown9(8)Tohidpour ACytotoxin-associated gene product A (CagA) is a major virulence factor secreted by <i>Helicobacter pylori</i>. CagA activity in the gastric epithelium is associated with higher risk of gastric cancer development. Bacterial type IV secretion system (T4SS)-mediated translocation of CagA into the cytosol of human epithelial cells occurs via a poorly understood mechanism that requires CagA interaction with the host membrane lipid phosphatidylserine (PS) and host cell receptor integrin α₅β₁. Here we have characterized the isolated recombinant middle fragment of CagA (CagA-M) that contains the positively-charged PS-binding region (aa 613-636) and a putative β₁ integrin binding site, but lacks the EPIYA region, secretion signal peptide and the CagA multimerization motif. We show that CagA-M, whenToxinsE237https://www.ebi.ac.uk/biostudies/studies/S-EPMC5577571biostudies-literatureThe Middle Fragment of Helicobacter pylori CagA Induces Actin Rearrangement and Triggers Its Own Uptake into Gastric Epithelial Cells.PMC5577571Roujeinikova AKwok TGorrell RJTohidpour AfalseThe Middle Fragment of Helicobacter pylori CagA Induces Actin Rearrangement and Triggers Its Own Uptake into Gastric Epithelial Cells.Cytotoxin-associated gene product A (CagA) is a major virulence factor secreted by <i>Helicobacter pylori</i>. CagA activity in the gastric epithelium is associated with higher risk of gastric cancer development. Bacterial type IV secretion system (T4SS)-mediated translocation of CagA into the cytosol of human epithelial cells occurs via a poorly understood mechanism that requires CagA interaction with the host membrane lipid phosphatidylserine (PS) and host cell receptor integrin α₅β₁. Here we have characterized the isolated recombinant middle fragment of CagA (CagA-M) that contains the positively-charged PS-binding region (aa 613-636) and a putative β₁ integrin binding site, but lacks the EPIYA region, secretion signal peptide and the CagA multimerization motif. We show that CagA-M, when2017-01-01T00:00:00Z2017 Jul2026-05-02T05:49:15.722Z2019-03-27T02:54:59ZS-EPMC55775712878807210.3390/toxins9080237