biostudies-literatureKumarasamy VUniversity Malaya High Impact Research Grante0183097https://www.ebi.ac.uk/biostudies/studies/S-EPMC5578604biostudies-literatureUnknown12(8)Colorectal cancer (CRC) is one the most commonly diagnosed cancers worldwide and the number is increasing every year. Despite advances in screening programs, CRC remains as the second leading cause of cancer deaths in the United States. Oxidative stress plays an important role in the molecular mechanisms of colorectal cancer (CRC) and has been shown to be associated with Blastocystis sp., a common intestinal microorganism. In the present study, we aimed to identify a role for Blastocystis sp. in exacerbating carcinogenesis using in vivo rat model. Methylene blue staining was used to identify colonic aberrant crypt foci (ACF) and adenomas formation in infected rats whilst elevation of oxidative stress biomarker levels in the urine and serum samples were evaluated using biochemical assays. HPloS oneExacerbation of colon carcinogenesis by Blastocystis sp.PMC5578604UM.C/625/1/HIR/MOHE/MED/44.Kumarasamy VSamudi CRagavan NDJayalakshmi PKuppusamy URKumar SfalseExacerbation of colon carcinogenesis by Blastocystis sp.Colorectal cancer (CRC) is one the most commonly diagnosed cancers worldwide and the number is increasing every year. Despite advances in screening programs, CRC remains as the second leading cause of cancer deaths in the United States. Oxidative stress plays an important role in the molecular mechanisms of colorectal cancer (CRC) and has been shown to be associated with Blastocystis sp., a common intestinal microorganism. In the present study, we aimed to identify a role for Blastocystis sp. in exacerbating carcinogenesis using in vivo rat model. Methylene blue staining was used to identify colonic aberrant crypt foci (ACF) and adenomas formation in infected rats whilst elevation of oxidative stress biomarker levels in the urine and serum samples were evaluated using biochemical assays. H2017-01-01T00:00:00Z20172025-04-26T11:14:35.163Z2019-03-27T02:55:03ZS-EPMC55786042885909510.1371/journal.pone.0183097