{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Frick PL"],"funding":["VICTR STARBRITE","NCRR NIH HHS","Uniting Against Lung Cancer","NCI NIH HHS","National Institutes of Health"],"pagination":["1403-12"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5580929"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["230(7)"],"pubmed_abstract":["The dynamics of heterogeneous clonal lineages within a cell population, in aggregate, shape both normal and pathological biological processes. Studies of clonality typically relate the fitness of clones to their relative abundance, thus requiring long-term experiments and limiting conclusions about the heterogeneity of clonal fitness in response to perturbation. We present, for the first time, a method that enables a dynamic, global picture of clonal fitness within a mammalian cell population. This novel assay allows facile comparison of the structure of clonal fitness in a cell population across many perturbations. By utilizing high-throughput imaging, our methodology provides ample statistical power to define clonal fitness dynamically and to visualize the structure of perturbation-induced clonal fitness within a cell population. We envision that this technique will be a powerful tool to investigate heterogeneity in biological processes involving cell proliferation, including development and drug response."],"journal":["Journal of cellular physiology"],"pubmed_title":["Quantifying heterogeneity and dynamics of clonal fitness in response to perturbation."],"pmcid":["PMC5580929"],"funding_grant_id":["R21 CA178589","NIH, UL1 RR024975-01","F31-CA165840","U54 CA113007","UL1 RR024975-01","13020513","5U54CA113007","F31 CA165840"],"pubmed_authors":["Tyson DR","Quaranta V","Paudel BB","Frick PL"],"additional_accession":[]},"is_claimable":false,"name":"Quantifying heterogeneity and dynamics of clonal fitness in response to perturbation.","description":"The dynamics of heterogeneous clonal lineages within a cell population, in aggregate, shape both normal and pathological biological processes. Studies of clonality typically relate the fitness of clones to their relative abundance, thus requiring long-term experiments and limiting conclusions about the heterogeneity of clonal fitness in response to perturbation. We present, for the first time, a method that enables a dynamic, global picture of clonal fitness within a mammalian cell population. This novel assay allows facile comparison of the structure of clonal fitness in a cell population across many perturbations. By utilizing high-throughput imaging, our methodology provides ample statistical power to define clonal fitness dynamically and to visualize the structure of perturbation-induced clonal fitness within a cell population. We envision that this technique will be a powerful tool to investigate heterogeneity in biological processes involving cell proliferation, including development and drug response.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Jul","modification":"2024-10-19T01:13:39.16Z","creation":"2019-03-27T02:55:12Z"},"accession":"S-EPMC5580929","cross_references":{"pubmed":["25600161"],"doi":["10.1002/jcp.24888"]}}