biostudies-literatureRieder LEAmerican Cancer SocietyPew Biomedical ScholarsNCI NIH HHSNational Institutes of HealthNIHNIGMS NIH HHS1494-1508https://www.ebi.ac.uk/biostudies/studies/S-EPMC5588930biostudies-literatureUnknown31(14)The conserved histone locus body (HLB) assembles prior to zygotic gene activation early during development and concentrates factors into a nuclear domain of coordinated histone gene regulation. Although HLBs form specifically at replication-dependent histone loci, the <i>cis</i> and <i>trans</i> factors that target HLB components to histone genes remained unknown. Here we report that conserved GA repeat <i>cis</i> elements within the bidirectional <i>histone3-histone4</i> promoter direct HLB formation in <i>Drosophila</i> In addition, the CLAMP (chromatin-linked adaptor for male-specific lethal [MSL] proteins) zinc finger protein binds these GA repeat motifs, increases chromatin accessibility, enhances histone gene transcription, and promotes HLB formation. We demonstrated previously that CLAMP also promotes the formation of another domain of coordinated gene regulation: the dosage-compensated male X chromosome. Therefore, CLAMP binding to GA repeat motifs promotes the formation of two distinct domains of coordinated gene activation located at different places in the genome.Genes & developmentHistone locus regulation by the <i>Drosophila</i> dosage compensation adaptor protein CLAMP.PMC5588930F32 GM109663GM058921T32 GM007092R01 GM098461123682-RSG-13-040-01-DMCR01 GM058921T32 CA009156GM098461Marzluff WFDuronio RJRieder LELarschan ENZeidman ATolstorukov MYKoreski KPBoltz KAJordan WTKuzu GUrban JABowman SKfalseHistone locus regulation by the <i>Drosophila</i> dosage compensation adaptor protein CLAMP.The conserved histone locus body (HLB) assembles prior to zygotic gene activation early during development and concentrates factors into a nuclear domain of coordinated histone gene regulation. Although HLBs form specifically at replication-dependent histone loci, the <i>cis</i> and <i>trans</i> factors that target HLB components to histone genes remained unknown. Here we report that conserved GA repeat <i>cis</i> elements within the bidirectional <i>histone3-histone4</i> promoter direct HLB formation in <i>Drosophila</i> In addition, the CLAMP (chromatin-linked adaptor for male-specific lethal [MSL] proteins) zinc finger protein binds these GA repeat motifs, increases chromatin accessibility, enhances histone gene transcription, and promotes HLB formation. We demonstrated previously that CLAMP also promotes the formation of another domain of coordinated gene regulation: the dosage-compensated male X chromosome. Therefore, CLAMP binding to GA repeat motifs promotes the formation of two distinct domains of coordinated gene activation located at different places in the genome.2017-01-01T00:00:00Z2017 Jul2024-11-12T04:07:46.622Z2019-03-27T02:55:43ZS-EPMC55889302883894610.1101/gad.300855.117