biostudies-literatureBertaux-Skeirik NBLRD VAUniversity of Cincinnati Graduate School Dean's FellowshipNational Institutes of HealthAlbert J Ryan FellowshipNational Institute of General Medical SciencesCollege of Medicine Bridge Funding ProgramDepartment of Veterans Affairs Merit Review AwardNIDDK NIH HHSPresbyterian Historical SocietyNCI NIH HHSNIAMS NIH HHSNational Cancer Institute of the National Institutes of HealthNIGMS NIH HHS463-475https://www.ebi.ac.uk/biostudies/studies/S-EPMC5591948biostudies-literatureUnknown242(4)The CD44 gene encodes several protein isoforms due to alternative splicing and post translational modifications. Given that CD44 variant isoform 9 (CD44v9) is expressed within Spasmolytic Polypeptide/TFF2-Expressing Metaplasia (SPEM) glands during repair, CD44v9 may be play a funcitonal role during the process of regeneration of the gastric epithelium. Here we hypothesize that CD44v9 marks a regenerative cell lineage responsive to infiltrating macrophages during regeneration of the gastric epithelium. Ulcers were induced in CD44-deficient (CD44KO) and C57BL/6 (BL6) mice by a localized application of acetic acid to the serosal surface of the stomach. Gastric organoids expressing CD44v9 were derived from mouse stomachs and transplanted at the ulcer site of CD44KO mice. Ulcers, CD44v9 expressThe Journal of pathologyCD44 variant isoform 9 emerges in response to injury and contributes to the regeneration of the gastric epithelium.PMC5591948R01 DK083402R50 CA211404P30 DK078392P30 DK058404I01 BX000930I01BX000930P30 AR047363R01 DK0715902 R01 DK083402-06T32 GM105526R50CA211404AR-47363T32 CA1061832T32GM105526-04Biesiada JSundaram NHawkins JYang LEngevik ACJian GWang JMahe MMedvedovic MChakrabarti JTeal EGoldenring JRMulloy JCQualls JEHelmrath MADiwan TZavros YWunderlich MBertaux-Skeirik NGabre JfalseCD44 variant isoform 9 emerges in response to injury and contributes to the regeneration of the gastric epithelium.The CD44 gene encodes several protein isoforms due to alternative splicing and post translational modifications. Given that CD44 variant isoform 9 (CD44v9) is expressed within Spasmolytic Polypeptide/TFF2-Expressing Metaplasia (SPEM) glands during repair, CD44v9 may be play a funcitonal role during the process of regeneration of the gastric epithelium. Here we hypothesize that CD44v9 marks a regenerative cell lineage responsive to infiltrating macrophages during regeneration of the gastric epithelium. Ulcers were induced in CD44-deficient (CD44KO) and C57BL/6 (BL6) mice by a localized application of acetic acid to the serosal surface of the stomach. Gastric organoids expressing CD44v9 were derived from mouse stomachs and transplanted at the ulcer site of CD44KO mice. Ulcers, CD44v9 express2017-01-01T00:00:00Z2017 Aug2025-04-19T08:43:14.851Z2019-03-27T02:55:58ZS-EPMC55919482849748410.1002/path.4918