biostudies-literatureUnknown7(1)Butenko IDespite the fact the term "proteome" was proposed to characterize a set of proteins in one of mycoplasma species, proteome response to various exposures in this bacteria are still obscure. Commonly, authors studying proteomic response on perturbation models in mycoplasmas use single approach and do not confirm their findings by alternative methods. Consequently, the results of proteomic analysis should be validated by complementary techniques. In this study we utilized three complementary approaches (SWATH, MRM, 2D-DIGE) to assess response of Mycoplasma gallisepticum under heat stress on proteomic level and combined these findings with metabolic response and the results of transcriptional profiling. We divide response into two modes - one is directly related to heat stress and other is triScientific reports11330https://www.ebi.ac.uk/biostudies/studies/S-EPMC5595898biostudies-literatureResponse induced in Mycoplasma gallisepticum under heat shock might be relevant to infection process.PMC5595898Vanyushkina AFisunov GMatyushkina DGovorun VGorbachev AAnikanov NPobeguts OButenko IKovalchuk SfalseResponse induced in Mycoplasma gallisepticum under heat shock might be relevant to infection process.Despite the fact the term "proteome" was proposed to characterize a set of proteins in one of mycoplasma species, proteome response to various exposures in this bacteria are still obscure. Commonly, authors studying proteomic response on perturbation models in mycoplasmas use single approach and do not confirm their findings by alternative methods. Consequently, the results of proteomic analysis should be validated by complementary techniques. In this study we utilized three complementary approaches (SWATH, MRM, 2D-DIGE) to assess response of Mycoplasma gallisepticum under heat stress on proteomic level and combined these findings with metabolic response and the results of transcriptional profiling. We divide response into two modes - one is directly related to heat stress and other is tri2017-01-01T00:00:00Z2017 Sep2026-05-06T03:42:45.954Z2019-03-27T02:56:09ZS-EPMC55958982890011610.1038/s41598-017-09237-7