biostudies-literatureUnknown5Martinez R<h4>Background</h4>Growth hormone secretagogues (GHS), among other factors, regulate the release of GH. The biological activity of the secretagogue peptide A233 as a promoter of growth and innate immunity in teleost fish has previously been demonstrated, but its role in the immune system of mammals is not well understood.<h4>Methods</h4>The effect of the peptide was investigated in J774A.2 macrophage cells using a comparative proteomics approach after 6 and 12 h of peptide stimulation.<h4>Results</h4>The functional analysis of differentially modulated proteins showed that A233 peptide treatment appears to promote activation and ROS-dependent cytotoxic functions in macrophages and enhanced expression of antiviral protein complexes such as MAVS. In accordance with this hypothesis, we found tBiochemistry and biophysics reports379-387https://www.ebi.ac.uk/biostudies/studies/S-EPMC5600417biostudies-literatureComparative proteomic analysis of growth hormone secretagogue A233 treatment of murine macrophage cells J774A.2 indicates it has a role in antiviral innate response.PMC5600417Gonzalez LJRamos YBesada Vde Villavicencio-Diaz TNAlbericio FMendez MVonasek EMarcos EHermida LMartinez RRodriguez EGonzalez LGGaray HSanchez ASanchez BMasforrol YEstrada MPFerro JNfalseComparative proteomic analysis of growth hormone secretagogue A233 treatment of murine macrophage cells J774A.2 indicates it has a role in antiviral innate response.<h4>Background</h4>Growth hormone secretagogues (GHS), among other factors, regulate the release of GH. The biological activity of the secretagogue peptide A233 as a promoter of growth and innate immunity in teleost fish has previously been demonstrated, but its role in the immune system of mammals is not well understood.<h4>Methods</h4>The effect of the peptide was investigated in J774A.2 macrophage cells using a comparative proteomics approach after 6 and 12 h of peptide stimulation.<h4>Results</h4>The functional analysis of differentially modulated proteins showed that A233 peptide treatment appears to promote activation and ROS-dependent cytotoxic functions in macrophages and enhanced expression of antiviral protein complexes such as MAVS. In accordance with this hypothesis, we found t2016-01-01T00:00:00Z2016 Mar2026-04-15T17:10:59.428Z2025-05-29T16:23:28.967ZS-EPMC56004172895584510.1016/j.bbrep.2016.01.008