{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang Y"],"funding":["National Natural Science Foundation of China"],"pagination":["2971413"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5603109"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2017"],"pubmed_abstract":["With the emerging role of umbilical cord blood-derived mesenchymal stem cells (hUCB-MSC) for bone regeneration and delivery of therapeutic proteins, there is an increasing need for effective gene delivery systems to modify such cells. mTAT, a TAT peptide sequence bearing histidine and cysteine residues, has been successfully used for intracellular gene delivery. Using a gWiz-GFP plasmid, we demonstrated that polyethylenimine combined with mTAT (mTAT/PEI) displayed good transfection efficacy in hUCB-MSC. hUCB-MSC transfected with mTAT/PEI were shown to express more BMP-2 protein and mRNA, indicating the feasibility of using the cells as a BMP-2 delivery system. Importantly, compared to PEI25, a \"gold standard\" nonviral transfection polymer, mTAT/PEI had limited toxicity to the cells. Furthe"],"journal":["BioMed research international"],"pubmed_title":["Modification of Human Umbilical Cord Blood Stem Cells Using Polyethylenimine Combined with Modified TAT Peptide to Enhance BMP-2 Production."],"pmcid":["PMC5603109"],"funding_grant_id":["2017-325","2015M541484","LBH-Z14147","81401342"],"pubmed_authors":["Li J","Yan J","Wei R","You C","Song C","Wang Y","Zu J"],"additional_accession":[]},"is_claimable":false,"name":"Modification of Human Umbilical Cord Blood Stem Cells Using Polyethylenimine Combined with Modified TAT Peptide to Enhance BMP-2 Production.","description":"With the emerging role of umbilical cord blood-derived mesenchymal stem cells (hUCB-MSC) for bone regeneration and delivery of therapeutic proteins, there is an increasing need for effective gene delivery systems to modify such cells. mTAT, a TAT peptide sequence bearing histidine and cysteine residues, has been successfully used for intracellular gene delivery. Using a gWiz-GFP plasmid, we demonstrated that polyethylenimine combined with mTAT (mTAT/PEI) displayed good transfection efficacy in hUCB-MSC. hUCB-MSC transfected with mTAT/PEI were shown to express more BMP-2 protein and mRNA, indicating the feasibility of using the cells as a BMP-2 delivery system. Importantly, compared to PEI25, a \"gold standard\" nonviral transfection polymer, mTAT/PEI had limited toxicity to the cells. Furthe","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2025-04-05T14:31:20.115Z","creation":"2019-03-27T02:56:40Z"},"accession":"S-EPMC5603109","cross_references":{"pubmed":["28951869"],"doi":["10.1155/2017/2971413"]}}