{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(9)"],"submitter":["Dos Santos Cavalcanti A"],"funding":["Pew Charitable Trusts"],"pubmed_abstract":["Osteosarcoma is the most common primary bone tumor in children and young adults. The median survival of osteosarcoma patients has not significantly improved since 1990, despite administration of different classes of chemotherapy agents, such as methotrexate, cisplatin and doxorubicin. Cancer stem cells (CSCs) are responsible for the resistance of osteosarcoma to chemotherapy and OCT4, SOX2 and SSEA4 have been used to identify CSCs in osteosarcoma. Here, we used low-passage patient-derived osteosarcoma cells and osteosarcoma cells directly isolated from patients before and after chemotherapy treatments to evaluate the effects of chemotherapy on stem cell markers expression. We demonstrate that primary osteosarcoma cells are resistant to methotrexate treatment and sensitive to cisplatin and "],"journal":["PloS one"],"pagination":["e0184891"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5608286"],"repository":["biostudies-literature"],"pubmed_title":["Patient-derived osteosarcoma cells are resistant to methotrexate."],"pmcid":["PMC5608286"],"pubmed_authors":["Leite Duarte ME","Gholamin S","Avan A","Kahn SA","Dos Santos Cavalcanti A","Ribeiro GO","de Sa Lopes AC","Meohas W","Razavi M","Hanae Kasai Brunswick T","Matheus Guimaraes JA"],"additional_accession":[]},"is_claimable":false,"name":"Patient-derived osteosarcoma cells are resistant to methotrexate.","description":"Osteosarcoma is the most common primary bone tumor in children and young adults. The median survival of osteosarcoma patients has not significantly improved since 1990, despite administration of different classes of chemotherapy agents, such as methotrexate, cisplatin and doxorubicin. Cancer stem cells (CSCs) are responsible for the resistance of osteosarcoma to chemotherapy and OCT4, SOX2 and SSEA4 have been used to identify CSCs in osteosarcoma. Here, we used low-passage patient-derived osteosarcoma cells and osteosarcoma cells directly isolated from patients before and after chemotherapy treatments to evaluate the effects of chemotherapy on stem cell markers expression. We demonstrate that primary osteosarcoma cells are resistant to methotrexate treatment and sensitive to cisplatin and ","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2026-05-06T06:32:25.024Z","creation":"2019-03-27T02:57:01Z"},"accession":"S-EPMC5608286","cross_references":{"pubmed":["28934267"],"doi":["10.1371/journal.pone.0184891"]}}