{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Banfi C"],"pubmed_abstract":["Analysis of the cellular proteome can help to elucidate the molecular mechanisms underlying diseases due to the development of technologies that permit the large-scale identification and quantification of the proteins present in complex biological systems.The knowledge gained from a proteomic approach can potentially lead to a better understanding of the pathogenic mechanisms underlying diseases, allowing for the identification of novel diagnostic and prognostic disease markers, and, hopefully, of therapeutic targets. However, the cardiac mitral valve represents a very challenging sample for proteomic analysis because of the low cellularity in proteoglycan and collagen-enriched extracellular matrix. This makes it challenging to extract proteins for a global proteomic analysis. This work de"],"journal":["Journal of visualized experiments : JoVE"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5608423"],"repository":["biostudies-literature"],"pubmed_title":["Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve."],"pmcid":["PMC5608423"],"pubmed_authors":["Tremoli E","Polvani G","Guarino A","Mastrullo V","Banfi C","Brioschi M","Ghilardi S"],"additional_accession":[]},"is_claimable":false,"name":"Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve.","description":"Analysis of the cellular proteome can help to elucidate the molecular mechanisms underlying diseases due to the development of technologies that permit the large-scale identification and quantification of the proteins present in complex biological systems.The knowledge gained from a proteomic approach can potentially lead to a better understanding of the pathogenic mechanisms underlying diseases, allowing for the identification of novel diagnostic and prognostic disease markers, and, hopefully, of therapeutic targets. However, the cardiac mitral valve represents a very challenging sample for proteomic analysis because of the low cellularity in proteoglycan and collagen-enriched extracellular matrix. This makes it challenging to extract proteins for a global proteomic analysis. This work de","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jun","modification":"2026-06-17T03:15:40.005Z","creation":"2020-11-19T16:52:02Z"},"accession":"S-EPMC5608423","cross_references":{"pubmed":["28654069"],"doi":["10.3791/55762"]}}