biostudies-literatureUnknownBanfi CAnalysis of the cellular proteome can help to elucidate the molecular mechanisms underlying diseases due to the development of technologies that permit the large-scale identification and quantification of the proteins present in complex biological systems.The knowledge gained from a proteomic approach can potentially lead to a better understanding of the pathogenic mechanisms underlying diseases, allowing for the identification of novel diagnostic and prognostic disease markers, and, hopefully, of therapeutic targets. However, the cardiac mitral valve represents a very challenging sample for proteomic analysis because of the low cellularity in proteoglycan and collagen-enriched extracellular matrix. This makes it challenging to extract proteins for a global proteomic analysis. This work deJournal of visualized experiments : JoVEhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC5608423biostudies-literatureOptimized Protocol for the Extraction of Proteins from the Human Mitral Valve.PMC5608423Tremoli EPolvani GGuarino AMastrullo VBanfi CBrioschi MGhilardi SfalseOptimized Protocol for the Extraction of Proteins from the Human Mitral Valve.Analysis of the cellular proteome can help to elucidate the molecular mechanisms underlying diseases due to the development of technologies that permit the large-scale identification and quantification of the proteins present in complex biological systems.The knowledge gained from a proteomic approach can potentially lead to a better understanding of the pathogenic mechanisms underlying diseases, allowing for the identification of novel diagnostic and prognostic disease markers, and, hopefully, of therapeutic targets. However, the cardiac mitral valve represents a very challenging sample for proteomic analysis because of the low cellularity in proteoglycan and collagen-enriched extracellular matrix. This makes it challenging to extract proteins for a global proteomic analysis. This work de2017-01-01T00:00:00Z2017 Jun2026-06-17T03:15:40.005Z2020-11-19T16:52:02ZS-EPMC56084232865406910.3791/55762