{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Reithmair M"],"funding":["German Federal Ministry of Economy and Energy"],"pagination":["2403-2411"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5618677"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(10)"],"pubmed_abstract":["Septic shock is a common medical condition with a mortality approaching 50% where early diagnosis and treatment are of particular importance for patient survival. Novel biomarkers that serve as prompt indicators of sepsis are urgently needed. High-throughput technologies assessing circulating microRNAs represent an important tool for biomarker identification, but the blood-compartment specificity of these miRNAs has not yet been investigated. We characterized miRNA profiles from serum exosomes, total serum and blood cells (leukocytes, erythrocytes, platelets) of sepsis patients by next-generation sequencing and RT-qPCR (n = 3 × 22) and established differences in miRNA expression between blood compartments. In silico analysis was used to identify compartment-specific signalling functions of"],"journal":["Journal of cellular and molecular medicine"],"pubmed_title":["Cellular and extracellular miRNAs are blood-compartment-specific diagnostic targets in sepsis."],"pmcid":["PMC5618677"],"funding_grant_id":["KF3361701MD4","WB1317"],"pubmed_authors":["Reithmair M","Pfob M","Schelling G","Buschmann D","Hagl D","Kaufmann I","Pfaffl MW","Marte M","Kirchner B","Chouker A","Steinlein OK"],"additional_accession":[]},"is_claimable":false,"name":"Cellular and extracellular miRNAs are blood-compartment-specific diagnostic targets in sepsis.","description":"Septic shock is a common medical condition with a mortality approaching 50% where early diagnosis and treatment are of particular importance for patient survival. Novel biomarkers that serve as prompt indicators of sepsis are urgently needed. High-throughput technologies assessing circulating microRNAs represent an important tool for biomarker identification, but the blood-compartment specificity of these miRNAs has not yet been investigated. We characterized miRNA profiles from serum exosomes, total serum and blood cells (leukocytes, erythrocytes, platelets) of sepsis patients by next-generation sequencing and RT-qPCR (n = 3 × 22) and established differences in miRNA expression between blood compartments. In silico analysis was used to identify compartment-specific signalling functions of","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Oct","modification":"2026-05-06T03:44:48.185Z","creation":"2019-03-27T02:57:37Z"},"accession":"S-EPMC5618677","cross_references":{"pubmed":["28382754"],"doi":["10.1111/jcmm.13162"]}}