{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["2(17)"],"submitter":["Priedigkeit N"],"pubmed_abstract":["Bone metastases (BoM) are a significant cause of morbidity in patients with estrogen receptor-positive (ER-positive) breast cancer; yet, characterizations of human specimens are limited. In this study, exome-capture RNA sequencing (ecRNA-seq) on aged (8-12 years), formalin-fixed, paraffin-embedded (FFPE), and decalcified cancer specimens was evaluated. Gene expression values and ecRNA-seq quality metrics from FFPE or decalcified tumor RNA showed minimal differences when compared with matched flash-frozen or nondecalcified tumors. ecRNA-seq was then applied on a longitudinal collection of 11 primary breast cancers and patient-matched synchronous or recurrent BoMs. Overtime, BoMs exhibited gene expression shifts to more Her2 and LumB PAM50 subtype profiles, temporally influenced expression e"],"journal":["JCI insight"],"pagination":["95703"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5621874"],"repository":["biostudies-literature"],"pubmed_title":["Exome-capture RNA sequencing of decade-old breast cancers and matched decalcified bone metastases."],"pmcid":["PMC5621874"],"pubmed_authors":["Lee AV","Basudan A","Lucas PC","Brufsky AM","Bhargava R","Oesterreich S","Watters RJ","Kolls JK","Priedigkeit N","Fang Z","Rosenzweig MQ","Horne W","Weiss KR"],"additional_accession":[]},"is_claimable":false,"name":"Exome-capture RNA sequencing of decade-old breast cancers and matched decalcified bone metastases.","description":"Bone metastases (BoM) are a significant cause of morbidity in patients with estrogen receptor-positive (ER-positive) breast cancer; yet, characterizations of human specimens are limited. In this study, exome-capture RNA sequencing (ecRNA-seq) on aged (8-12 years), formalin-fixed, paraffin-embedded (FFPE), and decalcified cancer specimens was evaluated. Gene expression values and ecRNA-seq quality metrics from FFPE or decalcified tumor RNA showed minimal differences when compared with matched flash-frozen or nondecalcified tumors. ecRNA-seq was then applied on a longitudinal collection of 11 primary breast cancers and patient-matched synchronous or recurrent BoMs. Overtime, BoMs exhibited gene expression shifts to more Her2 and LumB PAM50 subtype profiles, temporally influenced expression e","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Sep","modification":"2025-04-22T07:41:41.826Z","creation":"2019-03-27T02:57:48Z"},"accession":"S-EPMC5621874","cross_references":{"pubmed":["28878133"],"doi":["10.1172/jci.insight.95703"]}}