{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(1)"],"submitter":["Matsuura T"],"pubmed_abstract":["The Cys2/His2-type zinc finger protein Zfp296 has been implicated in stem cell pluripotency and tumor pathogenesis. However, its mechanisms remain elusive. Here, we demonstrated that a Zfp296 deficiency in mice impairs germ-cell development and embryonic growth. Zfp296 was intracellularly localized to heterochromatin in embryos. A GST-Zfp296 pull-down experiment using ES cell nuclear extract followed by LC-MS/MS showed that Zfp296 interacts with component proteins of heterochromatin (such as HP1, Dnmt1, Dnmt3b, and ATRX) and the NuRD complex. We focused on H3K9 methylation as a hallmark of heterochromatin, and found that Zfp296 overexpression in cultured cells reduces the Suv39h1-mediated H3K9 methylation. Consistent with this finding, in Zfp296 <sup>-/-</sup> mouse embryos, we observed a "],"journal":["Scientific reports"],"pagination":["12462"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5622089"],"repository":["biostudies-literature"],"pubmed_title":["Zfp296 negatively regulates H3K9 methylation in embryonic development as a component of heterochromatin."],"pmcid":["PMC5622089"],"pubmed_authors":["Miyazaki JI","Matsuura T","Tashiro F","Miyazaki T","Miyazaki S"],"additional_accession":[]},"is_claimable":false,"name":"Zfp296 negatively regulates H3K9 methylation in embryonic development as a component of heterochromatin.","description":"The Cys2/His2-type zinc finger protein Zfp296 has been implicated in stem cell pluripotency and tumor pathogenesis. However, its mechanisms remain elusive. Here, we demonstrated that a Zfp296 deficiency in mice impairs germ-cell development and embryonic growth. Zfp296 was intracellularly localized to heterochromatin in embryos. A GST-Zfp296 pull-down experiment using ES cell nuclear extract followed by LC-MS/MS showed that Zfp296 interacts with component proteins of heterochromatin (such as HP1, Dnmt1, Dnmt3b, and ATRX) and the NuRD complex. We focused on H3K9 methylation as a hallmark of heterochromatin, and found that Zfp296 overexpression in cultured cells reduces the Suv39h1-mediated H3K9 methylation. Consistent with this finding, in Zfp296 <sup>-/-</sup> mouse embryos, we observed a ","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Sep","modification":"2026-05-05T21:21:31.423Z","creation":"2019-03-27T02:57:52Z"},"accession":"S-EPMC5622089","cross_references":{"pubmed":["28963472"],"doi":["10.1038/s41598-017-12772-y"]}}