biostudies-literatureVuong NQOntario Graduate Scholarship in Science and TechnologyHealth CanadaOntario Graduate Scholarship39https://www.ebi.ac.uk/biostudies/studies/S-EPMC5625787biostudies-literatureUnknown14(1)<h4>Background</h4>Toxicity of airborne particulate matter (PM) is difficult to assess because PM composition is complex and variable due to source contribution and atmospheric transformation. In this study, we used an in vitro toxicoproteomic approach to identify the toxicity mechanisms associated with different subfractions of Ottawa urban dust (EHC-93).<h4>Methods</h4>A549 human lung epithelial cells were exposed to 0, 60, 140 and 200 μg/cm² doses of EHC-93 (total), its insoluble and soluble fractions for 24 h. Multiple cytotoxicity assays and proteomic analyses were used to assess particle toxicity in the exposed cells.<h4>Results</h4>The cytotoxicity data based on cellular ATP, BrdU incorporation and LDH leakage indicated that the insoluble, but not the soluble, fraction isParticle and fibre toxicologyIn vitro toxicoproteomic analysis of A549 human lung epithelial cells exposed to urban air particulate matter and its water-soluble and insoluble fractions.PMC5625787Clean Air Regulatory Agenda (Grant # 4340565)Breznan DKarthikeyan SKumarathasan PO'Brien JSWilliams AVincent RGoegan PVuong NQfalseIn vitro toxicoproteomic analysis of A549 human lung epithelial cells exposed to urban air particulate matter and its water-soluble and insoluble fractions.<h4>Background</h4>Toxicity of airborne particulate matter (PM) is difficult to assess because PM composition is complex and variable due to source contribution and atmospheric transformation. In this study, we used an in vitro toxicoproteomic approach to identify the toxicity mechanisms associated with different subfractions of Ottawa urban dust (EHC-93).<h4>Methods</h4>A549 human lung epithelial cells were exposed to 0, 60, 140 and 200 μg/cm² doses of EHC-93 (total), its insoluble and soluble fractions for 24 h. Multiple cytotoxicity assays and proteomic analyses were used to assess particle toxicity in the exposed cells.<h4>Results</h4>The cytotoxicity data based on cellular ATP, BrdU incorporation and LDH leakage indicated that the insoluble, but not the soluble, fraction is2017-01-01T00:00:00Z2017 Oct2025-04-26T08:32:46.893Z2019-03-27T02:58:03ZS-EPMC56257872896966310.1186/s12989-017-0220-6