biostudies-literatureMohamed AANIDDK NIH HHSNCI NIH HHSNIHCPDR-USU program1308-1317https://www.ebi.ac.uk/biostudies/studies/S-EPMC5626592biostudies-literatureUnknown15(10)The oncogenic activation of the ETS-related gene (<i>ERG</i>) due to gene fusions is present in over half of prostate cancers in Western countries. Because of its high incidence and oncogenic role, ERG and components of ERG network have emerged as potential drug targets for prostate cancer. Utilizing gene expression datasets, from matched normal and prostate tumor epithelial cells, an association of NOTCH transcription factors with <i>ERG</i> expression status was identified, confirming that <i>NOTCH</i> factors are direct transcriptional targets of ERG. Inhibition of ERG in <i>TMPRSS2-ERG</i>-positive VCaP cells led to decreased levels of NOTCH1 and 2 proteins and downstream transcriptional targets and partially recapitulated the phenotypes associated with ERG inhibition. Regulation of <iMolecular cancer research : MCRSynergistic Activity with NOTCH Inhibition and Androgen Ablation in ERG-Positive Prostate Cancer Cells.PMC5626592RO1 DK065977R01 CA162383R01 DK065977HU0001-10-2-0002Dobi ALi HXavier CPSreenath TLSrivatsan ESSrinivasan AKatta SJamal MSrivastava STan SHRavindranath LHuang WMohamed AAMcLeod DGPetrovics GSrivastava MfalseSynergistic Activity with NOTCH Inhibition and Androgen Ablation in ERG-Positive Prostate Cancer Cells.The oncogenic activation of the ETS-related gene (<i>ERG</i>) due to gene fusions is present in over half of prostate cancers in Western countries. Because of its high incidence and oncogenic role, ERG and components of ERG network have emerged as potential drug targets for prostate cancer. Utilizing gene expression datasets, from matched normal and prostate tumor epithelial cells, an association of NOTCH transcription factors with <i>ERG</i> expression status was identified, confirming that <i>NOTCH</i> factors are direct transcriptional targets of ERG. Inhibition of ERG in <i>TMPRSS2-ERG</i>-positive VCaP cells led to decreased levels of NOTCH1 and 2 proteins and downstream transcriptional targets and partially recapitulated the phenotypes associated with ERG inhibition. Regulation of <i2017-01-01T00:00:00Z2017 Oct2026-03-27T15:35:52.649Z2019-03-26T23:57:54ZS-EPMC56265922860700710.1158/1541-7786.mcr-17-005810.1158/1541-7786.MCR-17-0058