biostudies-literatureUnknown25(10)Gefen TSylvester Comprehensive Cancer Center, Medical School, University of MiamiDodson Interdisciplinary Immunotherapy InstituteT cell immunoglobulin-3 (TIM-3) is a negative regulator of interferon-γ (IFN-γ) secreting CD4⁺ T cells and CD8⁺ T cytotoxic cells. Recent studies have highlighted the role of TIM-3 as an important mediator of CD8⁺ T cell exhaustion in the setting of chronic viral infections and cancer. In murine tumor models, antibody blockade of TIM-3 with anti-TIM-3 antibodies as monotherapy has no or minimal antitumor activity, suggesting that TIM-3 signaling exerts an accessory or amplifying effect in keeping immune responses in check. Using a combined bead and cell-based systemic evolution of ligands by exponential enrichment (SELEX) protocol, we have isolated nuclease-resistant oligonucleotide aptamer ligands that bind to cell-associated TIM-3 with high affinity and sMolecular therapy : the journal of the American Society of Gene Therapy2280-2288https://www.ebi.ac.uk/biostudies/studies/S-EPMC5628791biostudies-literatureA TIM-3 Oligonucleotide Aptamer Enhances T Cell Functions and Potentiates Tumor Immunity in Mice.PMC5628791Puplampu-Dove YGefen TCastro IPatel SGilboa EMuharemagic DfalseA TIM-3 Oligonucleotide Aptamer Enhances T Cell Functions and Potentiates Tumor Immunity in Mice.T cell immunoglobulin-3 (TIM-3) is a negative regulator of interferon-γ (IFN-γ) secreting CD4⁺ T cells and CD8⁺ T cytotoxic cells. Recent studies have highlighted the role of TIM-3 as an important mediator of CD8⁺ T cell exhaustion in the setting of chronic viral infections and cancer. In murine tumor models, antibody blockade of TIM-3 with anti-TIM-3 antibodies as monotherapy has no or minimal antitumor activity, suggesting that TIM-3 signaling exerts an accessory or amplifying effect in keeping immune responses in check. Using a combined bead and cell-based systemic evolution of ligands by exponential enrichment (SELEX) protocol, we have isolated nuclease-resistant oligonucleotide aptamer ligands that bind to cell-associated TIM-3 with high affinity and s2017-01-01T00:00:00Z2017 Oct2025-04-20T03:39:13.921Z2019-03-26T23:57:55ZS-EPMC56287912880095410.1016/j.ymthe.2017.06.023