{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(39)"],"submitter":["Svobodova M"],"pubmed_abstract":["In this study, we describe the establishment of the human papillomavirus 18-positive, stage II, grade 1, T2N0M0 head and neck tumor primary cell line derived from oral squamous cell carcinoma of a non-smoking patient by using two different protocols. Furthermore, a preparation of subpopulations derived from this primary cell line according to the cluster of differentiation molecules CD44/CD90 status using magnetic bead-based separation and their characterization was performed. Impedance-based real-time cell analysis, enzyme-linked immunsorbant assay (ELISA), wound-healing assay, flow-cytometry, gene expression analysis, and MTT assay were used to characterize these four subpopulations (CD44<sup>+</sup>/CD90<sup>-</sup>, CD44<sup>-</sup>/CD90<sup>-</sup>, CD44<sup>+</sup>/CD90<sup>+</sup>, "],"journal":["Oncotarget"],"pagination":["66254-66269"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5630409"],"repository":["biostudies-literature"],"pubmed_title":["Establishment of oral squamous cell carcinoma cell line and magnetic bead-based isolation and characterization of its CD90/CD44 subpopulations."],"pmcid":["PMC5630409"],"pubmed_authors":["Balvan J","Polanska H","Kostrica R","Heger Z","Fojtu M","Svobodova M","Raudenska M","Gumulec J","Kratochvilova M","Masarik M","Horakova Z","Babula P"],"additional_accession":[]},"is_claimable":false,"name":"Establishment of oral squamous cell carcinoma cell line and magnetic bead-based isolation and characterization of its CD90/CD44 subpopulations.","description":"In this study, we describe the establishment of the human papillomavirus 18-positive, stage II, grade 1, T2N0M0 head and neck tumor primary cell line derived from oral squamous cell carcinoma of a non-smoking patient by using two different protocols. Furthermore, a preparation of subpopulations derived from this primary cell line according to the cluster of differentiation molecules CD44/CD90 status using magnetic bead-based separation and their characterization was performed. Impedance-based real-time cell analysis, enzyme-linked immunsorbant assay (ELISA), wound-healing assay, flow-cytometry, gene expression analysis, and MTT assay were used to characterize these four subpopulations (CD44<sup>+</sup>/CD90<sup>-</sup>, CD44<sup>-</sup>/CD90<sup>-</sup>, CD44<sup>+</sup>/CD90<sup>+</sup>, ","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Sep","modification":"2026-07-16T13:25:30.158Z","creation":"2019-03-27T02:58:23Z"},"accession":"S-EPMC5630409","cross_references":{"pubmed":["29029509"],"doi":["10.18632/oncotarget.19914"]}}