{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mei H"],"funding":["NEI NIH HHS","National Eye Institute","Research to Prevent Blindness","California Institute for Regenerative Medicine","National Eye Institute grants"],"pagination":["e0186238"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5636133"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(10)"],"pubmed_abstract":["The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells. To reduce possible xenobiotic contamination from 3T3s, primary human adipose-derived stem cells (ASCs) were examined as feeder cells to support the expansion of LSCs in vitro. To optimize the ASC-supported culture, freshly isolated limbal epithelial cells in the form of single cells (SC-ASC) or cell clusters (CC-ASC) were cultured using three different methods: LSCs seeded directly on feeder cells, a 3-dimensional (3D) culture system and a 3D culture system with fibrin (fibrin 3D). The expanded LSCs were examined at the end of a 2-week culture. The standard 3T3 culture served as control. Expansion of SC-ASC show"],"journal":["PloS one"],"pubmed_title":["Human adipose-derived stem cells support the growth of limbal stem/progenitor cells."],"pmcid":["PMC5636133"],"funding_grant_id":["1R01EY021797","R01 EY021797","5P30EY000331","P30 EY000331","TR2-01768"],"pubmed_authors":["Nakatsu MN","Mei H","Deng SX","Chen FV","Gonzalez S","Baclagon ER"],"additional_accession":[]},"is_claimable":false,"name":"Human adipose-derived stem cells support the growth of limbal stem/progenitor cells.","description":"The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells. To reduce possible xenobiotic contamination from 3T3s, primary human adipose-derived stem cells (ASCs) were examined as feeder cells to support the expansion of LSCs in vitro. To optimize the ASC-supported culture, freshly isolated limbal epithelial cells in the form of single cells (SC-ASC) or cell clusters (CC-ASC) were cultured using three different methods: LSCs seeded directly on feeder cells, a 3-dimensional (3D) culture system and a 3D culture system with fibrin (fibrin 3D). The expanded LSCs were examined at the end of a 2-week culture. The standard 3T3 culture served as control. Expansion of SC-ASC show","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2025-04-26T20:05:46.429Z","creation":"2019-03-27T02:58:41Z"},"accession":"S-EPMC5636133","cross_references":{"pubmed":["29020119"],"doi":["10.1371/journal.pone.0186238"]}}