biostudies-literatureUnknown81(4)Moon JYA <i>Salmonella</i> Typhimurium ghost vaccine was constructed with the use of a recombinant fusion protein consisting of lysozyme and porcine myeloid antimicrobial peptide 36 expressed by the <i>Escherichia coli</i> overexpression system. After confirmation of its effectiveness by transmission electron microscopy the vaccine was evaluated in a murine model. Of the 60 BALB/c mice equally divided into 4 groups, group A mice were intramuscularly inoculated with 100 μL of sterile phosphate-buffered saline, and the mice in groups B, C, and D were intramuscularly inoculated with approximately 1.0 × 10⁴, 1.0 × 10⁵, or 1.0 × 10⁶ cells of the <i>S.</i> Typhimurium ghost vaccine, respectively, in 100-μL amounts. The serum IgG titers against <i>S.</i> Typhimurium outeCanadian journal of veterinary research = Revue canadienne de recherche veterinaire297-303https://www.ebi.ac.uk/biostudies/studies/S-EPMC5644453biostudies-literatureProtective efficacy of a <i>Salmonella</i> Typhimurium ghost vaccine candidate constructed with a recombinant lysozyme-PMAP36 fusion protein in a murine model.PMC5644453Kim WKMun JYKim SYHur JKim BPark JHMoon JYChoi HSRao ZfalseProtective efficacy of a <i>Salmonella</i> Typhimurium ghost vaccine candidate constructed with a recombinant lysozyme-PMAP36 fusion protein in a murine model.A <i>Salmonella</i> Typhimurium ghost vaccine was constructed with the use of a recombinant fusion protein consisting of lysozyme and porcine myeloid antimicrobial peptide 36 expressed by the <i>Escherichia coli</i> overexpression system. After confirmation of its effectiveness by transmission electron microscopy the vaccine was evaluated in a murine model. Of the 60 BALB/c mice equally divided into 4 groups, group A mice were intramuscularly inoculated with 100 μL of sterile phosphate-buffered saline, and the mice in groups B, C, and D were intramuscularly inoculated with approximately 1.0 × 10⁴, 1.0 × 10⁵, or 1.0 × 10⁶ cells of the <i>S.</i> Typhimurium ghost vaccine, respectively, in 100-μL amounts. The serum IgG titers against <i>S.</i> Typhimurium oute2017-01-01T00:00:00Z2017 Oct2025-06-01T03:24:19.624Z2021-02-20T02:02:22ZS-EPMC564445329081588