biostudies-literatureClements DRNova Scotia Health Research FoundationTerry Fox Research InstituteNIDDK NIH HHSNational Institute of Diabetes and Digestive and Kidney DiseasesBeatrice Hunter Cancer Research InstituteWellcome TrustCanadian Institutes of Health ResearchCanadian Cancer Society Research Institute3391-3406https://www.ebi.ac.uk/biostudies/studies/S-EPMC5648240biostudies-literatureUnknown16(9)Myeloid cells play a central role in the context of viral eradication, yet precisely how these cells differentiate throughout the course of acute infections is poorly understood. In this study, we have developed a novel quantitative temporal in vivo proteomics (QTiPs) platform to capture proteomic signatures of temporally transitioning virus-driven myeloid cells directly in situ, thus taking into consideration host-virus interactions throughout the course of an infection. QTiPs, in combination with phenotypic, functional, and metabolic analyses, elucidated a pivotal role for inflammatory CD11b⁺, Ly6G^-, Ly6C^high-low cells in antiviral immune response and viral clearance. Most importantly, the time-resolved QTiPs data set showed the transition of CD11b<sup>+<Journal of proteome researchQuantitative Temporal in Vivo Proteomics Deciphers the Transition of Virus-Driven Myeloid Cells into M2 Macrophages.PMC5648240108070/Z/15/ZK01 DK098285Holay NGujar SKennedy BESterea AWeekes MPClements DRKonda PSharif TAlmasi SLee PWHelson EPaulo JAGygi SPMurphy JPKim YfalseQuantitative Temporal in Vivo Proteomics Deciphers the Transition of Virus-Driven Myeloid Cells into M2 Macrophages.Myeloid cells play a central role in the context of viral eradication, yet precisely how these cells differentiate throughout the course of acute infections is poorly understood. In this study, we have developed a novel quantitative temporal in vivo proteomics (QTiPs) platform to capture proteomic signatures of temporally transitioning virus-driven myeloid cells directly in situ, thus taking into consideration host-virus interactions throughout the course of an infection. QTiPs, in combination with phenotypic, functional, and metabolic analyses, elucidated a pivotal role for inflammatory CD11b⁺, Ly6G^-, Ly6C^high-low cells in antiviral immune response and viral clearance. Most importantly, the time-resolved QTiPs data set showed the transition of CD11b<sup>+<2017-01-01T00:00:00Z2017 Sep2026-05-04T23:13:44.69Z2026-04-07T21:03:20.213ZS-EPMC56482402876841410.1021/acs.jproteome.7b00425