{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Syrett CM"],"funding":["National Institute of Allergy and Infectious Diseases","NICHD NIH HHS","Lupus Foundation of America","NIAID NIH HHS","National Institute of General Medical Sciences","National Institute of Child Health and Human Development","NIGMS NIH HHS"],"pagination":["e1007050"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5648283"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(10)"],"pubmed_abstract":["X-chromosome inactivation (XCI) in female lymphocytes is uniquely regulated, as the inactive X (Xi) chromosome lacks localized Xist RNA and heterochromatin modifications. Epigenetic profiling reveals that Xist RNA is lost from the Xi at the pro-B cell stage and that additional heterochromatic modifications are gradually lost during B cell development. Activation of mature B cells restores Xist RNA and heterochromatin to the Xi in a dynamic two-step process that differs in timing and pattern, depending on the method of B cell stimulation. Finally, we find that DNA binding domain of YY1 is necessary for XCI in activated B cells, as ex-vivo YY1 deletion results in loss of Xi heterochromatin marks and up-regulation of X-linked genes. Ectopic expression of the YY1 zinc finger domain is sufficie"],"journal":["PLoS genetics"],"pubmed_title":["Loss of Xist RNA from the inactive X during B cell development is restored in a dynamic YY1-dependent two-step process in activated B cells."],"pmcid":["PMC5648283"],"funding_grant_id":["RO1 GM111384","RO1 AI079002","T32-GM007229","T32-HD083185","K12 HD085848","R01 AI079002","R21 AI124084","HD085848","T32 GM007229","T32 HD083185","R01 GM111384"],"pubmed_authors":["Sindhava V","Anguera MC","Syrett CM","Myles A","Liang G","Cancro M","Hodawadekar S","Zhang Y","Atchison M","Nandi S"],"additional_accession":[]},"is_claimable":false,"name":"Loss of Xist RNA from the inactive X during B cell development is restored in a dynamic YY1-dependent two-step process in activated B cells.","description":"X-chromosome inactivation (XCI) in female lymphocytes is uniquely regulated, as the inactive X (Xi) chromosome lacks localized Xist RNA and heterochromatin modifications. Epigenetic profiling reveals that Xist RNA is lost from the Xi at the pro-B cell stage and that additional heterochromatic modifications are gradually lost during B cell development. Activation of mature B cells restores Xist RNA and heterochromatin to the Xi in a dynamic two-step process that differs in timing and pattern, depending on the method of B cell stimulation. Finally, we find that DNA binding domain of YY1 is necessary for XCI in activated B cells, as ex-vivo YY1 deletion results in loss of Xi heterochromatin marks and up-regulation of X-linked genes. Ectopic expression of the YY1 zinc finger domain is sufficie","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Oct","modification":"2026-04-08T02:46:31.876Z","creation":"2026-04-07T21:02:56.487Z"},"accession":"S-EPMC5648283","cross_references":{"pubmed":["28991910"],"doi":["10.1371/journal.pgen.1007050"]}}