biostudies-literatureLiang HNCI NIH HHSNIGMS NIH HHS7260-7264https://www.ebi.ac.uk/biostudies/studies/S-EPMC5650247biostudies-literatureUnknown8(39)A promising biosensor for effectively lead (II) ion detection in practical applications was developed by constructing a Pb²⁺-specific L-DNAzyme, the enantiomer of the natural nucleic acid-constructed D-DNAzyme. This fluorescent sensor contains the L-enzyme strand with a quencher at the 3' end, and the L-substrate strand with a fluorophore at the 5' and a quencher at the 3' ends that formed a complex. In the presence of Pb²⁺, the L-substrate is cut into two fragments, leading to the recovery of fluorescence. The sensor shows high sensitivity and selectivity for Pb²⁺ detection with a linear response in the range of 5-100 nM and a detection limit of 3 nM in aqueous solution. Importantly, based on that L-DNAzyme consists of non-natural nucleic acids, which is iAnalytical methods : advancing methods and applicationsDesigning a Biostable L-DNAzyme for Lead(II) Ion Detection in Practical Samples.PMC5650247R01 CA133086R01 GM079359Liang HCui LZhang XWu CXie SfalseDesigning a Biostable L-DNAzyme for Lead(II) Ion Detection in Practical Samples.A promising biosensor for effectively lead (II) ion detection in practical applications was developed by constructing a Pb²⁺-specific L-DNAzyme, the enantiomer of the natural nucleic acid-constructed D-DNAzyme. This fluorescent sensor contains the L-enzyme strand with a quencher at the 3' end, and the L-substrate strand with a fluorophore at the 5' and a quencher at the 3' ends that formed a complex. In the presence of Pb²⁺, the L-substrate is cut into two fragments, leading to the recovery of fluorescence. The sensor shows high sensitivity and selectivity for Pb²⁺ detection with a linear response in the range of 5-100 nM and a detection limit of 3 nM in aqueous solution. Importantly, based on that L-DNAzyme consists of non-natural nucleic acids, which is i2016-01-01T00:00:00Z2016 Oct2025-04-26T08:37:12.54Z2019-03-27T02:59:30ZS-EPMC56502472906239010.1039/C6AY01791F10.1039/c6ay01791f